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- W1528990718 abstract "1 Baker's yeast histones have been isolated either by acid extraction of purified chromatin or by salt-dissociation of crude chromatin. These two methods gave similar results as judged by the amino-acid composition and electrophoretic behaviour of histones. 2 Chemical fractionation methods have been used to isolate both, moderately lysine-rich and arginine-rich histones. Neither F1 nor F3 histones could be detected in yeast chromatin throughout these fractionation procedures. Results obtained in experiments of interactions between yeast whole histones ans calf thymus DNA also confirm the absence of a very lysine-rich histone in yeast. 3 The fractionation of arginine-rich histones was achieved by selective precipitation with acetone. F2A1 and F2A2 histones were obtained, purified and characterised by electrophoresis and amino acid analyses. The similarities between yeast and other organisms histone fractions are discussed." @default.
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- W1528990718 date "1974-06-01" @default.
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- W1528990718 title "Histones from Baker's Yeast. Isolation and Fractionation" @default.
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- W1528990718 doi "https://doi.org/10.1111/j.1432-1033.1974.tb03532.x" @default.
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