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- W1529126852 abstract "This chapter focuses on the bacterial artificial chromosome (BAC) clones and the current clone map of the zebrafish genome. The genome project that is undertaken for the zebrafish combines three branches. The first is the maintenance and refinement of the genetic map and markers. The second is the generation of a fully contiguated physical map of the chromosomes by using bacterial artificial chromosome (BAC) clones. The third is the sequencing of the whole genome by whole-genome shotgun sequencing (WGS) and sequencing of a minimal set of overlapping clones from the physical map. The chapter describes a few strategies that combine the available repositories and can help the zebrafish researcher solve biological questions. It also gives an example of how to identify the zebrafish dicer1 gene making use of the physical map and BAC libraries. This method can be altered depending on what information is available for gene. To find out on which BAC clones the region of interest is located, one can use two strategies: hybridization to spotted BAC filters or screening pools that are generated from the libraries through PCR. Hybridization is mostly achieved by labeling a specific probe for the region of interest and incubation with the filters to allow hybridization to the positive BAC clones. The current physical map being generated from fingerprinted clones can be a valuable resource for zebrafish researchers. In combination with other available resources and tools, ordered clones may function as a good starting point to examine the genomic organization of genes in zebrafish." @default.
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- W1529126852 date "2004-01-01" @default.
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- W1529126852 title "Bacterial Artificial Chromosome (BAC) Clones and the Current Clone Map of the Zebrafish Genome" @default.
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- W1529126852 doi "https://doi.org/10.1016/s0091-679x(04)77016-8" @default.
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