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• W1529127577 endingPage "7743" @default.
• W1529127577 startingPage "7735" @default.
• W1529127577 abstract "The p107 protein is related to the Rb protein by a 60-kDa region of homology called the pocket domain that binds cellular proteins such as E2F and cyclins A and E as well as the viral oncoproteins E1A, E7, and SV40 T antigen. The p107 and Rb proteins have both been implicated as negative regulators of cell growth. We have examined the effect of the unphosphorylated pocket domain of p107 on specific stages of the T antigen-mediated replication of SV40 DNA in vitro. The pocket domain inhibited replication by preventing the assembly of T antigen at the SV40 core origin DNA containing binding site II. However, both proteins formed a ternary complex with DNA containing T antigen-binding site I. The pocket domain of p107 did not inhibit the oligomerization of T antigen in the absence of DNA, and the p107 derivative is bound to the intermediates of this reaction. In the unwinding assay, once T antigen was preassembled as hexamers at the core origin, the pocket domain bound to and stabilized the complex, resulting in an increase in the yield of unwound product. Preformed T antigen hexamers complexed with the pocket domain bind to a synthetic replication fork, and this complex supported unwinding. Consistent with this, the p107 pocket domain had no effect on the helicase activity of T antigen in an assay using a partial duplex substrate. However, a complex containing p107 and T antigen assembled at the core origin did not support SV40 DNA replication in HeLa cell crude extracts or in the monopolymerase reaction. This inhibition is due to the inability of the complex to bind to DNA polymerase alpha, which is required for the initiation of DNA synthesis. The data suggest pathways by which the pocket domain of p107 can negatively regulate T antigen-mediated replication in vitro. The binding of T antigen to p107 is discussed with respect to its role in mitigating negative cell growth control, resulting in viral mediated transformation." @default.
• W1529127577 created "2016-06-24" @default.
• W1529127577 creator A5013700795 @default.
• W1529127577 creator A5030497198 @default.
• W1529127577 creator A5078778212 @default.
• W1529127577 date "1994-03-01" @default.
• W1529127577 modified "2023-09-29" @default.
• W1529127577 title "Initiation of DNA replication by simian virus 40 T antigen is inhibited by the p107 protein." @default.
• W1529127577 cites W1221384716 @default.
• W1529127577 cites W14762771 @default.
• W1529127577 cites W1484137762 @default.
• W1529127577 cites W1490364597 @default.
• W1529127577 cites W1493331137 @default.
• W1529127577 cites W1508702670 @default.
• W1529127577 cites W1516321864 @default.
• W1529127577 cites W1517952165 @default.
• W1529127577 cites W1527058604 @default.
• W1529127577 cites W1544564404 @default.
• W1529127577 cites W1544570479 @default.
• W1529127577 cites W1554307469 @default.
• W1529127577 cites W1558614105 @default.
• W1529127577 cites W1559981481 @default.
• W1529127577 cites W1560152642 @default.
• W1529127577 cites W1578842363 @default.
• W1529127577 cites W1587132435 @default.
• W1529127577 cites W1590134197 @default.
• W1529127577 cites W1598352287 @default.
• W1529127577 cites W1605567462 @default.
• W1529127577 cites W1625430625 @default.
• W1529127577 cites W1632041306 @default.
• W1529127577 cites W1640638881 @default.
• W1529127577 cites W1676148024 @default.
• W1529127577 cites W1764343287 @default.
• W1529127577 cites W1772887764 @default.
• W1529127577 cites W1965168699 @default.
• W1529127577 cites W1966340370 @default.
• W1529127577 cites W1972653649 @default.
• W1529127577 cites W1979300852 @default.
• W1529127577 cites W1979383311 @default.
• W1529127577 cites W1979837932 @default.
• W1529127577 cites W1981004390 @default.
• W1529127577 cites W1982622947 @default.
• W1529127577 cites W1982933686 @default.
• W1529127577 cites W1985324055 @default.
• W1529127577 cites W1988939915 @default.
• W1529127577 cites W1989075626 @default.
• W1529127577 cites W1989969910 @default.
• W1529127577 cites W1993552911 @default.
• W1529127577 cites W1994840092 @default.
• W1529127577 cites W1995806503 @default.
• W1529127577 cites W2012293213 @default.
• W1529127577 cites W2014222675 @default.
• W1529127577 cites W2015446431 @default.
• W1529127577 cites W2017293277 @default.
• W1529127577 cites W2024387238 @default.
• W1529127577 cites W2027532032 @default.
• W1529127577 cites W2035762638 @default.
• W1529127577 cites W2039653473 @default.
• W1529127577 cites W2046085471 @default.
• W1529127577 cites W2049221234 @default.
• W1529127577 cites W2050932117 @default.
• W1529127577 cites W2053432671 @default.
• W1529127577 cites W2055162988 @default.
• W1529127577 cites W2058090614 @default.
• W1529127577 cites W2059110341 @default.
• W1529127577 cites W2059594509 @default.
• W1529127577 cites W2062810379 @default.
• W1529127577 cites W2066980099 @default.
• W1529127577 cites W2078066154 @default.
• W1529127577 cites W2080087666 @default.
• W1529127577 cites W2085295693 @default.
• W1529127577 cites W2086693806 @default.
• W1529127577 cites W2087333644 @default.
• W1529127577 cites W2091158533 @default.
• W1529127577 cites W2094619757 @default.
• W1529127577 cites W2094692189 @default.
• W1529127577 cites W2114738441 @default.
• W1529127577 cites W2134331780 @default.
• W1529127577 cites W2135890914 @default.
• W1529127577 cites W2137059153 @default.
• W1529127577 cites W2137249911 @default.
• W1529127577 cites W2139047630 @default.
• W1529127577 cites W2139768549 @default.
• W1529127577 cites W2155009866 @default.
• W1529127577 cites W2159511823 @default.
• W1529127577 cites W2169154093 @default.
• W1529127577 cites W81266651 @default.
• W1529127577 cites W2061788718 @default.
• W1529127577 doi "https://doi.org/10.1016/s0021-9258(17)37348-9" @default.
• W1529127577 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8126000" @default.
• W1529127577 hasPublicationYear "1994" @default.
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