FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1529177558> ?p ?o ?g. }

• W1529177558 endingPage "4319" @default.
• W1529177558 startingPage "4307" @default.
• W1529177558 abstract "Salt-inducible kinase-1 (SIK1) is phosphorylated at Ser577 by protein kinase A in adrenocorticotropic hormone-stimulated Y1 cells, and the phospho-SIK1 translocates from the nucleus to the cytoplasm. The phospho-SIK1 is dephosphorylated in the cytoplasm and re-enters the nucleus several hours later. By using green-fluorescent protein-tagged SIK1 fragments, we found that a peptide region (586-612) was responsible for the nuclear localization of SIK1. The region was named the 'RK-rich region' because of its Arg- and Lys-rich nature. SIK1s mutated in the RK-rich region were localized mainly in the cytoplasm. Because SIK1 represses cAMP-response element (CRE)-mediated transcription of steroidogenic genes, the mutants were examined for their effect on transcription. To our surprise, the cytoplasmic mutants strongly repressed the CRE-binding protein (CREB) activity, the extent of repression being similar to that of SIK1(S577A), a mutant localized exclusively in the nucleus. Several chimeras were constructed from SIK1 and from its isoform SIK2, which was localized mainly in the cytoplasm, and they were examined for intracellular localization as well as CREB-repression activity. A SIK1-derived chimera, where the RK-rich region had been replaced with the corresponding region of SIK2, was found in the cytoplasm, its CREB-modulating activity being similar to that of wild-type SIK1. On the other hand, a SIK2-derived chimera with the RK-rich region of SIK1 was localized in both the nucleus and the cytoplasm, and had a CREB-repressing activity similar to that of the wild-type SIK2. Green fluorescent protein-fused transducer of regulated CREB activity 2 (TORC2), a CREB-specific co-activator, was localized in the cytoplasm and nucleus of Y1 cells, and, after treatment with adrenocorticotropic hormone, cytoplasmic TORC2 entered the nucleus, activating CREB. The SIK1 mutants, having a strong CRE-repressing activity, completely inhibited the adrenocorticotropic hormone-induced nuclear entry of green fluorescent protein-fused TORC2. This suggests that SIK1 may regulate the intracellular movement of TORC2, and as a result modulates the CREB-dependent transcription activity. Together, these results indicate that the RK-rich region of SIK1 is important for determining the nuclear localization and attenuating CREB-repressing activity, but the degree of the nuclear localization of SIK1 itself does not necessarily reflect the degree of SIK1-mediated CREB repression." @default.
• W1529177558 created "2016-06-24" @default.
• W1529177558 creator A5031128573 @default.
• W1529177558 creator A5044706467 @default.
• W1529177558 creator A5072363463 @default.
• W1529177558 creator A5075261929 @default.
• W1529177558 creator A5082185893 @default.
• W1529177558 creator A5087774085 @default.
• W1529177558 creator A5036609580 @default.
• W1529177558 date "2004-10-25" @default.
• W1529177558 modified "2023-09-26" @default.
• W1529177558 title "Salt-inducible kinase-1 represses cAMP response element-binding protein activity both in the nucleus and in the cytoplasm" @default.
• W1529177558 cites W1558785883 @default.
• W1529177558 cites W1562998360 @default.
• W1529177558 cites W1565979608 @default.
• W1529177558 cites W1608322029 @default.
• W1529177558 cites W1944794732 @default.
• W1529177558 cites W1964337986 @default.
• W1529177558 cites W1966720607 @default.
• W1529177558 cites W1972781375 @default.
• W1529177558 cites W1977180767 @default.
• W1529177558 cites W1978104702 @default.
• W1529177558 cites W1979270142 @default.
• W1529177558 cites W1979906352 @default.
• W1529177558 cites W1982284863 @default.
• W1529177558 cites W1984728203 @default.
• W1529177558 cites W1989553571 @default.
• W1529177558 cites W1990654724 @default.
• W1529177558 cites W2001443225 @default.
• W1529177558 cites W2004735731 @default.
• W1529177558 cites W2004753972 @default.
• W1529177558 cites W2008922773 @default.
• W1529177558 cites W2009661936 @default.
• W1529177558 cites W2009975411 @default.
• W1529177558 cites W2017284082 @default.
• W1529177558 cites W2027838253 @default.
• W1529177558 cites W2027874507 @default.
• W1529177558 cites W2027981450 @default.
• W1529177558 cites W2028242823 @default.
• W1529177558 cites W2029371986 @default.
• W1529177558 cites W2033529039 @default.
• W1529177558 cites W2033722975 @default.
• W1529177558 cites W2045987655 @default.
• W1529177558 cites W2051577598 @default.
• W1529177558 cites W2058211761 @default.
• W1529177558 cites W2060088582 @default.
• W1529177558 cites W2062362728 @default.
• W1529177558 cites W2065634344 @default.
• W1529177558 cites W2066163812 @default.
• W1529177558 cites W2067099248 @default.
• W1529177558 cites W2072398256 @default.
• W1529177558 cites W2079990775 @default.
• W1529177558 cites W2081524299 @default.
• W1529177558 cites W2084429276 @default.
• W1529177558 cites W2087697646 @default.
• W1529177558 cites W2088359631 @default.
• W1529177558 cites W2121060827 @default.
• W1529177558 cites W2123194197 @default.
• W1529177558 cites W2124281485 @default.
• W1529177558 cites W2124432707 @default.
• W1529177558 cites W2138319716 @default.
• W1529177558 cites W2139334062 @default.
• W1529177558 cites W2145330934 @default.
• W1529177558 cites W2158941552 @default.
• W1529177558 cites W2163031867 @default.
• W1529177558 cites W2168007569 @default.
• W1529177558 cites W2168780144 @default.
• W1529177558 cites W2184203459 @default.
• W1529177558 doi "https://doi.org/10.1111/j.1432-1033.2004.04372.x" @default.
• W1529177558 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15511237" @default.
• W1529177558 hasPublicationYear "2004" @default.
• W1529177558 type Work @default.
• W1529177558 sameAs 1529177558 @default.
• W1529177558 citedByCount "72" @default.
• W1529177558 countsByYear W15291775582012 @default.
• W1529177558 countsByYear W15291775582013 @default.
• W1529177558 countsByYear W15291775582014 @default.
• W1529177558 countsByYear W15291775582015 @default.
• W1529177558 countsByYear W15291775582016 @default.
• W1529177558 countsByYear W15291775582017 @default.
• W1529177558 countsByYear W15291775582018 @default.
• W1529177558 countsByYear W15291775582019 @default.
• W1529177558 countsByYear W15291775582020 @default.
• W1529177558 countsByYear W15291775582022 @default.
• W1529177558 countsByYear W15291775582023 @default.
• W1529177558 crossrefType "journal-article" @default.
• W1529177558 hasAuthorship W1529177558A5031128573 @default.
• W1529177558 hasAuthorship W1529177558A5036609580 @default.
• W1529177558 hasAuthorship W1529177558A5044706467 @default.
• W1529177558 hasAuthorship W1529177558A5072363463 @default.
• W1529177558 hasAuthorship W1529177558A5075261929 @default.
• W1529177558 hasAuthorship W1529177558A5082185893 @default.
• W1529177558 hasAuthorship W1529177558A5087774085 @default.
• W1529177558 hasConcept C104317684 @default.
• W1529177558 hasConcept C143065580 @default.
• W1529177558 hasConcept C150194340 @default.
• W1529177558 hasConcept C153911025 @default.
• W1529177558 hasConcept C1629964 @default.

• next