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• W1529239864 endingPage "60" @default.
• W1529239864 startingPage "49" @default.
• W1529239864 abstract "1. The acyl-CoA:lysophosphatidylcholine acyltransferase system in rat lung microsomes was found to utilize selectively 1-[1-14C]palmitoyl-sn-glycero-3-phosphocholine when compared with 1-[9,10-3H2]stearoyl-sn-glycero-3-phosphocholine. This result was found with either palmitoyl-CoA, linoleoyl-CoA or an equimolar mixture of these acyl donors and confirms recent data reported by Holub, Piekarski and Possmayer (Can. J. Biochem. 58 (1980) 434-439). 2. The selective utilization of palmitoyl lysophosphatidylcholine from a mixture of lysophosphatidylcholine species may cause an increased isotopic ratio in phosphatidylcholine when compared with that of total lysophosphatidylcholine. Thus, when rats were injected with a single doubly labelled species, i.e. 1-[9,10-3H2]palmitoyl-sn-glycero-3-phospho[methyl-14C]choline, the isotopic ratio in both total and disaturated phosphatidylcholine from lung was nearly identical to that of the injected substrate. This suggested a direct acylation by lung acyl-CoA:lysophosphatidylcholine acyltransferases. By contrast, when a mixture of 1-[9,10-3H2]palmitoyl-sn-glycero-3-phospho[methyl-14C]choline and 1-stearoyl-sn-glycero-3-phospho[methyl-14C]choline was injected, the 3H/14C ratio in disaturated lung phosphatidylcholine increased to about 1.4-fold that of the injected substrate. 3. These data indicate that increased isotopic ratios in disaturated phosphatidylcholine of lung tissue, after intravenous injection of lysophosphatidylcholine, do not necessarily point to the involvement of lysophosphatidylcholine:lysophosphatidylcholine transacylase in disaturated phosphatidylcholine formation." @default.
• W1529239864 created "2016-06-24" @default.
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• W1529239864 date "1981-04-01" @default.
• W1529239864 modified "2023-09-25" @default.
• W1529239864 title "Selective utilization of palmitoyl lysophosphatidylcholine in the synthesis of disaturated phosphatidylcholine in rat lung" @default.
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• W1529239864 doi "https://doi.org/10.1016/0005-2760(81)90027-8" @default.
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