FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1529549063> ?p ?o ?g. }

• W1529549063 endingPage "14746" @default.
• W1529549063 startingPage "14740" @default.
• W1529549063 abstract "Abstract Reconstituted proteoliposomes containing functional Neurospora crassa plasma membrane H(+)-ATPase molecules oriented predominantly with their cytoplasmic surface exposed were treated with trypsin and then subjected to Sepharose CL-6B column chromatography to remove the liberated peptides. The peptides remaining associated with the liposomes were then separated from the phospholipid by Sephadex LH-60 column chromatography and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Six H(+)-ATPase peptides with approximate molecular masses of 7, 7.5, 8, 10, 14, and 21 kDa were found to be tightly associated with the liposomal membrane. Amino acid sequencing of the 7-, 7.5-, and 21-kDa peptides in the LH-60 eluate identified them as H(+)-ATPase fragments beginning at residues 99 or 100, 272, and 660, respectively. After further purification, the approximately 10- and 14-kDa peptides were also similarly identified as beginning at residues 272 and 660. The approximately 8-kDa fragment was purified further but could not be sequenced, presumably indicating NH2-terminal blockage. To identify which of the liposome-associated peptides are embedded in the membrane, H(+)-ATPase molecules in the proteoliposomes were labeled from the hydrophobic membrane interior with 3-(trifluoromethyl)-3-(m-[125I]iodophenyl)diazirine and cleaved with trypsin, after which the membrane-associated peptides were purified and assessed for the presence of label. The results indicate that the approximately 7-, 7.5-, and 21-kDa peptides are in contact with the lipid bilayer whereas the approximately 8-kDa peptide is not. Taken together with the results of our recent analyses of the peptides released from the proteoliposomes, this information establishes the transmembrane topography of nearly all of the 919 residues in the H(+)-ATPase molecule." @default.
• W1529549063 created "2016-06-24" @default.
• W1529549063 creator A5027438376 @default.
• W1529549063 creator A5042461464 @default.
• W1529549063 creator A5049159831 @default.
• W1529549063 date "1991-08-01" @default.
• W1529549063 modified "2023-09-27" @default.
• W1529549063 title "Identification of the membrane-embedded regions of the Neurospora crassa plasma membrane H(+)-ATPase" @default.
• W1529549063 cites W126980359 @default.
• W1529549063 cites W1500101066 @default.
• W1529549063 cites W1508103020 @default.
• W1529549063 cites W1541048010 @default.
• W1529549063 cites W1552224269 @default.
• W1529549063 cites W1574318499 @default.
• W1529549063 cites W1826595938 @default.
• W1529549063 cites W1932052686 @default.
• W1529549063 cites W1975032872 @default.
• W1529549063 cites W2007948620 @default.
• W1529549063 cites W2010416376 @default.
• W1529549063 cites W2011175576 @default.
• W1529549063 cites W2020955090 @default.
• W1529549063 cites W203636523 @default.
• W1529549063 cites W2041820412 @default.
• W1529549063 cites W2054572135 @default.
• W1529549063 cites W2056183698 @default.
• W1529549063 cites W2061233882 @default.
• W1529549063 cites W2071219823 @default.
• W1529549063 cites W2086106892 @default.
• W1529549063 cites W2092197701 @default.
• W1529549063 cites W2119460480 @default.
• W1529549063 cites W2137183838 @default.
• W1529549063 cites W2182056816 @default.
• W1529549063 doi "https://doi.org/10.1016/s0021-9258(18)98749-1" @default.
• W1529549063 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1830591" @default.
• W1529549063 hasPublicationYear "1991" @default.
• W1529549063 type Work @default.
• W1529549063 sameAs 1529549063 @default.
• W1529549063 citedByCount "37" @default.
• W1529549063 crossrefType "journal-article" @default.
• W1529549063 hasAuthorship W1529549063A5027438376 @default.
• W1529549063 hasAuthorship W1529549063A5042461464 @default.
• W1529549063 hasAuthorship W1529549063A5049159831 @default.
• W1529549063 hasBestOaLocation W15295490631 @default.
• W1529549063 hasConcept C104317684 @default.
• W1529549063 hasConcept C143065580 @default.
• W1529549063 hasConcept C181199279 @default.
• W1529549063 hasConcept C185592680 @default.
• W1529549063 hasConcept C23265538 @default.
• W1529549063 hasConcept C2776739539 @default.
• W1529549063 hasConcept C2776769606 @default.
• W1529549063 hasConcept C41625074 @default.
• W1529549063 hasConcept C55493867 @default.
• W1529549063 hasConcept C86803240 @default.
• W1529549063 hasConceptScore W1529549063C104317684 @default.
• W1529549063 hasConceptScore W1529549063C143065580 @default.
• W1529549063 hasConceptScore W1529549063C181199279 @default.
• W1529549063 hasConceptScore W1529549063C185592680 @default.
• W1529549063 hasConceptScore W1529549063C23265538 @default.
• W1529549063 hasConceptScore W1529549063C2776739539 @default.
• W1529549063 hasConceptScore W1529549063C2776769606 @default.
• W1529549063 hasConceptScore W1529549063C41625074 @default.
• W1529549063 hasConceptScore W1529549063C55493867 @default.
• W1529549063 hasConceptScore W1529549063C86803240 @default.
• W1529549063 hasIssue "22" @default.
• W1529549063 hasLocation W15295490631 @default.
• W1529549063 hasLocation W15295490632 @default.
• W1529549063 hasOpenAccess W1529549063 @default.
• W1529549063 hasPrimaryLocation W15295490631 @default.
• W1529549063 hasRelatedWork W148081301 @default.
• W1529549063 hasRelatedWork W1577273279 @default.
• W1529549063 hasRelatedWork W1902608271 @default.
• W1529549063 hasRelatedWork W1995742566 @default.
• W1529549063 hasRelatedWork W2003856206 @default.
• W1529549063 hasRelatedWork W2065484340 @default.
• W1529549063 hasRelatedWork W2072167131 @default.
• W1529549063 hasRelatedWork W289420462 @default.
• W1529549063 hasRelatedWork W4254694833 @default.
• W1529549063 hasRelatedWork W4290210744 @default.
• W1529549063 hasVolume "266" @default.
• W1529549063 isParatext "false" @default.
• W1529549063 isRetracted "false" @default.
• W1529549063 magId "1529549063" @default.
• W1529549063 workType "article" @default.