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- W1529604459 abstract "Breath analysis has potential to become a new medical diagnostic modality. In this thesis, a method for the analysis of human expired breath was developed using gas chromatography-mass spectroscopy. It was subsequently adopted for gas chromatography-differential mobility spectroscopy, a modality not previously applied to this problem. Tedlar bags and solid-phase microextraction were used for breath sampling and concentration prior to analysis. Four fiber coatings were evaluated with respect to selectivity and sensitivity; extraction time, gas chromatography temperature programming, and sample storage stability were explored for optimization. The method entails extraction and preconcentration with a polydimethylsiloxane-divinylbenzene coated fiber for 30 min at 37°C, and extraction profiles for several compounds demonstrate competitive adsorption. 120 compounds were identified in breath with response variability between 23 117% about mean values. Feasibility of differential mobility spectroscopy for breath analysis was established, and this method will be the basis for future investigations on the diagnostic potential of breath analysis. Technical Supervisor: Dr. Cristina E Davis Title: Principal Member of the Technical Staff; Group Leader, Bioengineering Thesis Advisor: Dr. Julie E Greenberg Title: Principal Research Scientist, Research Laboratory of Electronics" @default.
- W1529604459 created "2016-06-24" @default.
- W1529604459 creator A5058869043 @default.
- W1529604459 date "2005-01-01" @default.
- W1529604459 modified "2023-09-27" @default.
- W1529604459 title "Characterization of human expired breath by solid phase microextraction and analysis using gas chromatography-mass spectrometry and differential mobility spectrometry" @default.
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