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- W1529937658 abstract "Publisher Summary With the emergence of the polymerase chain reaction (PCR), considerable attention has been focused on isolating and characterizing the DNA polymerases of thermophiles. Numerous laboratory techniques, including PCR, RT-PCR (reverse transcription-PCR), isothermal amplification, DNA sequencing, mutagenesis, and cloning, rely on thermostable DNA polymerases for carrying out DNA synthesis in vitro at high temperatures. DNA polymerases perform essential roles in the replication and repair of genetic material. In addition to thermostable eubacterial enzymes, a number of DNA polymerases from hyperthermophilic archaea have been isolated and characterized. The most abundant DNA polymerase(s) in archaea are homologs of Family B DNA polymerases. This chapter presents a compilation of methods used to assay various activities associated with thermostable DNA polymerases, including DNA polymerase activity (nucleotide incorporation), processivity, strand displacement, and exonuclease activity. The chapter describes modifications to the nucleotide incorporation assay that allow measurements of thermostability, steady-state kinetic parameters, nucleotide analog incorporation, and reverse transcriptase activity. In addition, the chapter provides a brief discussion regarding polymerase error rate determinations." @default.
- W1529937658 created "2016-06-24" @default.
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- W1529937658 date "2001-01-01" @default.
- W1529937658 modified "2023-10-18" @default.
- W1529937658 title "[9] DNA polymerases from hyperthermophiles" @default.
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- W1529937658 doi "https://doi.org/10.1016/s0076-6879(01)34461-0" @default.
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