Matches in SemOpenAlex for { <https://semopenalex.org/work/W1530921056> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W1530921056 endingPage "3218" @default.
- W1530921056 startingPage "3212" @default.
- W1530921056 abstract "Abstract The La (SS-B) polypeptide is a ribonucleoprotein against which high titer antinuclear antibodies (ANA) react in the human autoimmune disease primary Sjögren's syndrome. To identify the autoepitopes with which the ANA anti-La (anti-SS-B) reacts, we isolated a 1.4-kb cDNA clone for La from a lambda gt10 library made from a human Burkitt's cell line. This clone contained an open reading frame of 1065 bp, encoding a 40.1-kDa polypeptide that corresponded to the carboxyl-terminal end of the La protein. The predicted polypeptide sequence of the recombinant protein was highly charged and unrelated to any previously published sequence. We also compared this clone to a previously published cDNA sequence for La and demonstrated significant differences, particularly that the open reading frame in our cDNA continued for 926 additional bases 3' to a putative termination codon in the previously reported sequence. The recombinant La protein was expressed in Escherichia coli and tested for reactivity with 200 sera containing ANA of various specificities. Only the sera containing anti-La antibodies reacted with the cloned La. By expressing subclones of the La cDNA as fusion proteins with beta-galactosidase, we have localized at least one epitope for the binding of anti-La antibodies to the carboxyl-terminal 103 amino acids of the La protein. No anti-La binding could be demonstrated to the region of the La protein that had previously been predicted to contain an autoepitope for the binding of anti-La (SS-B) antibodies. Studies of cloned autoepitopes could provide important clues to the role ANA play in disease and lead to targeted intervention in the treatment of primary Sjögren's syndrome." @default.
- W1530921056 created "2016-06-24" @default.
- W1530921056 creator A5000892381 @default.
- W1530921056 creator A5016565634 @default.
- W1530921056 creator A5040452583 @default.
- W1530921056 creator A5058046254 @default.
- W1530921056 creator A5078222317 @default.
- W1530921056 date "1988-05-01" @default.
- W1530921056 modified "2023-10-17" @default.
- W1530921056 title "Characteristics and epitope mapping of a cloned human autoantigen La." @default.
- W1530921056 doi "https://doi.org/10.4049/jimmunol.140.9.3212" @default.
- W1530921056 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2452201" @default.
- W1530921056 hasPublicationYear "1988" @default.
- W1530921056 type Work @default.
- W1530921056 sameAs 1530921056 @default.
- W1530921056 citedByCount "36" @default.
- W1530921056 countsByYear W15309210562015 @default.
- W1530921056 crossrefType "journal-article" @default.
- W1530921056 hasAuthorship W1530921056A5000892381 @default.
- W1530921056 hasAuthorship W1530921056A5016565634 @default.
- W1530921056 hasAuthorship W1530921056A5040452583 @default.
- W1530921056 hasAuthorship W1530921056A5058046254 @default.
- W1530921056 hasAuthorship W1530921056A5078222317 @default.
- W1530921056 hasBestOaLocation W15309210561 @default.
- W1530921056 hasConcept C104317684 @default.
- W1530921056 hasConcept C123894998 @default.
- W1530921056 hasConcept C153911025 @default.
- W1530921056 hasConcept C159654299 @default.
- W1530921056 hasConcept C167625842 @default.
- W1530921056 hasConcept C187882448 @default.
- W1530921056 hasConcept C189482833 @default.
- W1530921056 hasConcept C195616568 @default.
- W1530921056 hasConcept C2781241564 @default.
- W1530921056 hasConcept C40767141 @default.
- W1530921056 hasConcept C47289529 @default.
- W1530921056 hasConcept C54355233 @default.
- W1530921056 hasConcept C55493867 @default.
- W1530921056 hasConcept C81089528 @default.
- W1530921056 hasConcept C86803240 @default.
- W1530921056 hasConceptScore W1530921056C104317684 @default.
- W1530921056 hasConceptScore W1530921056C123894998 @default.
- W1530921056 hasConceptScore W1530921056C153911025 @default.
- W1530921056 hasConceptScore W1530921056C159654299 @default.
- W1530921056 hasConceptScore W1530921056C167625842 @default.
- W1530921056 hasConceptScore W1530921056C187882448 @default.
- W1530921056 hasConceptScore W1530921056C189482833 @default.
- W1530921056 hasConceptScore W1530921056C195616568 @default.
- W1530921056 hasConceptScore W1530921056C2781241564 @default.
- W1530921056 hasConceptScore W1530921056C40767141 @default.
- W1530921056 hasConceptScore W1530921056C47289529 @default.
- W1530921056 hasConceptScore W1530921056C54355233 @default.
- W1530921056 hasConceptScore W1530921056C55493867 @default.
- W1530921056 hasConceptScore W1530921056C81089528 @default.
- W1530921056 hasConceptScore W1530921056C86803240 @default.
- W1530921056 hasIssue "9" @default.
- W1530921056 hasLocation W15309210561 @default.
- W1530921056 hasLocation W15309210562 @default.
- W1530921056 hasOpenAccess W1530921056 @default.
- W1530921056 hasPrimaryLocation W15309210561 @default.
- W1530921056 hasRelatedWork W1530921056 @default.
- W1530921056 hasRelatedWork W2029042089 @default.
- W1530921056 hasRelatedWork W2037408474 @default.
- W1530921056 hasRelatedWork W2059246267 @default.
- W1530921056 hasRelatedWork W2083958575 @default.
- W1530921056 hasRelatedWork W2272103447 @default.
- W1530921056 hasRelatedWork W2369379504 @default.
- W1530921056 hasRelatedWork W2372950376 @default.
- W1530921056 hasRelatedWork W2396824485 @default.
- W1530921056 hasRelatedWork W2416984126 @default.
- W1530921056 hasVolume "140" @default.
- W1530921056 isParatext "false" @default.
- W1530921056 isRetracted "false" @default.
- W1530921056 magId "1530921056" @default.
- W1530921056 workType "article" @default.