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- W153172947 abstract "ABSTRACT. Using polyacrylamide disc gel electrophoresis, we examined the isozyme profiles of alkaline phosphatase in four human cell lines and determined the effect of hormone treatment. The isozymes of alkaline phosphatase were identified on the basis of their inhibition properties, heat stability, and cross reactivity to specific antisera. HeLa TCRC-1, a cell line monophenotypic for the Regan isozyme, and HeLa TCRC-2, monophenotypic for non-Regan alkaline phosphatase, each had a single specific band of enzyme activity. F1 amnion and HEp-2 cell lines each possessed their own characteristic isozyme pattern containing two bands of activity. We observed a marked alteration in the isozyme profiles after 72 hours of growth in prednisolone containing media. The Fl amnion, HEp-2, and HeLa TCRC-1 all showed an elevation of enzyme activity at the Regan migration position. The HEp-2 cell line exhibited a band at that position, which was enhanced by hormone treatment. The Fl amnion did not show a band at the Regan position; however, hormone treatment caused its induction. The HeLa TCRC-1 band of enzyme activity was significantly enhanced by hormone treatment. The enhanced band in all cell lines was shown to have all the properties of the Regan isozyme. Since HeLa TCRC-2 did not respond to hormone treatment, it seems that hormone enhancement is restricted to those cell lines which are producing or have the potential to produce the Regan isozyme. In addition to enhancing the Regan isozyme, this hormone was shown to diminish an intestinal component which was present in the Fl amnion and HEp-2 cell lines. In the lines studied, it appears that prednisolone regulates alkaline phosphatase activity by altering the activities of different isozymes." @default.
- W153172947 created "2016-06-24" @default.
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- W153172947 date "1975-01-01" @default.
- W153172947 modified "2023-09-25" @default.
- W153172947 title "SPECIFIC ISOZYME PROFILES OF ALKALINE PHOSPHATASE IN PREDNISOLONE-TREATED HUMAN CELL POPULATIONS" @default.
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- W153172947 doi "https://doi.org/10.1016/b978-0-12-472703-8.50051-2" @default.
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