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- W1531750550 abstract "crtYB, encoding lycopene cyclase and phytoene synthase was cloned from Rhodosporidium diobovatum ATCC 2527 by rapid amplification of cDNA ends method. The full-length cDNA of crtYB is 2, 330 bp and contains eight introns. The gene products is a 594 amino acids, with a predicted molecular mass of 65.63 kDa and a pI of 6.73. The N-terminus of the protein contains six transmembrane regions, which has been characterized as a lycopene beta-cyclase. The C-terminal half has squalene and phytoene synthase signatures that identified as phytoene synthetase. By heterologous complementary detection of this gene in E. coli and HPLC analysis, the regions responsible for phytoene synthesis and lycopene cyclization were localized within the protein." @default.
- W1531750550 created "2016-06-24" @default.
- W1531750550 creator A5063040162 @default.
- W1531750550 creator A5067028141 @default.
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- W1531750550 date "2014-03-27" @default.
- W1531750550 modified "2023-09-27" @default.
- W1531750550 title "Lycopene cyclase and phytoene synthase activities in the marine yeastRhodosporidium diobovatumare encoded by a single genecrtYB" @default.
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- W1531750550 doi "https://doi.org/10.1002/jobm.201300920" @default.
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