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- W1531924225 abstract "Large-scale cell death occurred when cotton suspension cells were treated with high concentration of cytokinin(2 mg·L~(-1)KT or 4 mg·L~(-1)KT),and the suspension cultures turned brown-black.Agarose gel electrophoresis of genomic DNA showed that apparent DNA fragmentations of multimers of 140-180 bp existed in the cells treated with high concentration of cytokinin(2 mg·L~(-1)KT or 4 mg·L~(-1)KT).Cytological studies also revealed that this kind of cell death was accompanied by such morphological characters as chromatin condenses,the maintaining of membrane continuity and condensed cytoplasm(shrinkage of the plasma membrane away from cell wall).These results supported that cell death induced by high concentration of cytokinin was a kind of PCD.In the experiment,we also found that cells in different physiological states(UCSA or BAE) and cells in various growth-phases(the exponential growth-phase or the quiescent phase) responded differentially to high concentration of cytokinin.The UCSA cells and the cells in exponential growth-phase were more sensitive to the induer." @default.
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- W1531924225 date "2005-01-01" @default.
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- W1531924225 title "High concentration of cytokinin induces programmed cell death of cotton suspension cultures." @default.
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