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• W1531994029 abstract "Charge pulse relaxation studies were performed on cells of the giant marine alga Valonia utricularis. Two exponential voltage relaxations were recorded as found previously (Benz, R., and U. Zimmermann. 1983. Biophys. J. 43:13-26.). The parameters of the two exponential voltage decays were studied as a function of the chloride concentration in the artificial sea water. Replacement of external chloride by 2(N-morpholino)ethanesulfonate (Mes(-)) had a dramatic influence on the four relaxation parameters. This chloride dependence could not be satisfactorily explained by the simplified model used earlier. Accordingly, additional reaction steps had to be included in the model. Only two relaxation processes could be resolved under all experimental conditions. This means that the heterogeneous complexation reactions, k(R) (association), and k(D) (dissociation) were too fast to be resolved. Therefore a carrier model with equilibrium heterogeneous surface reactions was used to fit the experimental results. From the charge pulse data at different chloride concentrations the translocation rate constants of the free and complexed carriers, k(S) and k(AS), through the membrane, as well as the total surface concentration of carrier systems, N(0), could be evaluated. The results described here indicate that the cell membrane of Valonia utricularis contains an electrogenic transport system for chloride." @default.
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• W1531994029 date "1991-01-01" @default.
• W1531994029 modified "2023-10-16" @default.
• W1531994029 title "Influence of external chloride concentration on the kinetics of mobile charges in the cell membrane of Valonia utricularis" @default.
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• W1531994029 doi "https://doi.org/10.1016/s0006-3495(91)82214-7" @default.
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