Matches in SemOpenAlex for { <https://semopenalex.org/work/W1532384213> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W1532384213 endingPage "411" @default.
- W1532384213 startingPage "405" @default.
- W1532384213 abstract "We have quantified, in cultured rat fibroblasts, the association to the lysosomal membrane of two classical plasma membrane markers, 5'-nucleotidase and alkaline phosphodiesterase I. To isolate highly purified lysosomal preparations, lysosomes were loaded with horseradish peroxidase (2-h cell uptake, 16-h chase) and isolated by isopycnic centrifugation in linear Percoll gradients, followed by a 3,3'-diaminobenzidine-induced density shift in sucrose gradients. Purified lysosomal preparations contained up to 50% of N-acetyl-beta-glucosaminidase of the homogenate. This lysosomal enzyme was enriched 33-fold in the most purified preparations. In the electron microscope, these preparations appeared to be highly purified and only contained organelles filled with diaminobenzidine reaction products. Analysis of purified preparations indicates that 0.5-0.8% of 5'-nucleotidase, but as much as 10.9-14.3% of alkaline phosphodiesterase I activities of the homogenate, are associated with lysosomes. After freezing-thawing, these activities remained essentially membrane-associated. The larger value obtained for alkaline phosphodiesterase I could not be ascribed to other lysosomal enzymes, as no such activity was detected at acidic pH. These two plasma membrane markers are thus unevenly distributed in the lysosomal compartment." @default.
- W1532384213 created "2016-06-24" @default.
- W1532384213 creator A5054539089 @default.
- W1532384213 creator A5070312706 @default.
- W1532384213 creator A5077055662 @default.
- W1532384213 creator A5087338225 @default.
- W1532384213 date "1987-12-01" @default.
- W1532384213 modified "2023-09-27" @default.
- W1532384213 title "Relations between plasma membrane and lysosomal membrane. 2. Quantitative evaluation of plasma membrane marker enzymes in the lysosomes" @default.
- W1532384213 cites W1582080277 @default.
- W1532384213 cites W1791904161 @default.
- W1532384213 cites W1973209022 @default.
- W1532384213 cites W1983622273 @default.
- W1532384213 cites W1988361400 @default.
- W1532384213 cites W1994975118 @default.
- W1532384213 cites W199871210 @default.
- W1532384213 cites W2019004530 @default.
- W1532384213 cites W2034900623 @default.
- W1532384213 cites W2060843203 @default.
- W1532384213 cites W2064435123 @default.
- W1532384213 cites W2075756072 @default.
- W1532384213 cites W2075846958 @default.
- W1532384213 cites W2078831219 @default.
- W1532384213 cites W2079635489 @default.
- W1532384213 cites W2092437540 @default.
- W1532384213 cites W2124536575 @default.
- W1532384213 cites W2153042101 @default.
- W1532384213 cites W2160107586 @default.
- W1532384213 cites W2417981236 @default.
- W1532384213 doi "https://doi.org/10.1111/j.1432-1033.1987.tb13714.x" @default.
- W1532384213 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2826161" @default.
- W1532384213 hasPublicationYear "1987" @default.
- W1532384213 type Work @default.
- W1532384213 sameAs 1532384213 @default.
- W1532384213 citedByCount "21" @default.
- W1532384213 crossrefType "journal-article" @default.
- W1532384213 hasAuthorship W1532384213A5054539089 @default.
- W1532384213 hasAuthorship W1532384213A5070312706 @default.
- W1532384213 hasAuthorship W1532384213A5077055662 @default.
- W1532384213 hasAuthorship W1532384213A5087338225 @default.
- W1532384213 hasBestOaLocation W15323842131 @default.
- W1532384213 hasConcept C132946820 @default.
- W1532384213 hasConcept C153911025 @default.
- W1532384213 hasConcept C160160445 @default.
- W1532384213 hasConcept C168240541 @default.
- W1532384213 hasConcept C181199279 @default.
- W1532384213 hasConcept C185592680 @default.
- W1532384213 hasConcept C18880352 @default.
- W1532384213 hasConcept C2777676563 @default.
- W1532384213 hasConcept C2780216420 @default.
- W1532384213 hasConcept C41625074 @default.
- W1532384213 hasConcept C43617362 @default.
- W1532384213 hasConcept C48679268 @default.
- W1532384213 hasConcept C55493867 @default.
- W1532384213 hasConcept C62826618 @default.
- W1532384213 hasConcept C7623868 @default.
- W1532384213 hasConcept C86803240 @default.
- W1532384213 hasConceptScore W1532384213C132946820 @default.
- W1532384213 hasConceptScore W1532384213C153911025 @default.
- W1532384213 hasConceptScore W1532384213C160160445 @default.
- W1532384213 hasConceptScore W1532384213C168240541 @default.
- W1532384213 hasConceptScore W1532384213C181199279 @default.
- W1532384213 hasConceptScore W1532384213C185592680 @default.
- W1532384213 hasConceptScore W1532384213C18880352 @default.
- W1532384213 hasConceptScore W1532384213C2777676563 @default.
- W1532384213 hasConceptScore W1532384213C2780216420 @default.
- W1532384213 hasConceptScore W1532384213C41625074 @default.
- W1532384213 hasConceptScore W1532384213C43617362 @default.
- W1532384213 hasConceptScore W1532384213C48679268 @default.
- W1532384213 hasConceptScore W1532384213C55493867 @default.
- W1532384213 hasConceptScore W1532384213C62826618 @default.
- W1532384213 hasConceptScore W1532384213C7623868 @default.
- W1532384213 hasConceptScore W1532384213C86803240 @default.
- W1532384213 hasIssue "1-2" @default.
- W1532384213 hasLocation W15323842131 @default.
- W1532384213 hasLocation W15323842132 @default.
- W1532384213 hasOpenAccess W1532384213 @default.
- W1532384213 hasPrimaryLocation W15323842131 @default.
- W1532384213 hasRelatedWork W1532384213 @default.
- W1532384213 hasRelatedWork W1974089542 @default.
- W1532384213 hasRelatedWork W1991442017 @default.
- W1532384213 hasRelatedWork W2022293381 @default.
- W1532384213 hasRelatedWork W2076258203 @default.
- W1532384213 hasRelatedWork W2078247653 @default.
- W1532384213 hasRelatedWork W2158537601 @default.
- W1532384213 hasRelatedWork W2402184542 @default.
- W1532384213 hasRelatedWork W4230029885 @default.
- W1532384213 hasRelatedWork W75802141 @default.
- W1532384213 hasVolume "170" @default.
- W1532384213 isParatext "false" @default.
- W1532384213 isRetracted "false" @default.
- W1532384213 magId "1532384213" @default.
- W1532384213 workType "article" @default.