FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1532698890> ?p ?o ?g. }

• W1532698890 endingPage "e0132848" @default.
• W1532698890 startingPage "e0132848" @default.
• W1532698890 abstract "Many therapeutic antibodies have been developed, and IgG antibodies have been extensively generated in various cell expression systems. IgG antibodies contain N-glycans at the constant region of the heavy chain (Fc domain), and their N-glycosylation patterns differ during various processes or among cell expression systems. The Fc N-glycan can modulate the effector functions of IgG antibodies, such as antibody-dependent cellular cytotoxicity (ADCC) and complement dependent cytotoxicity (CDC). To control Fc N-glycans, we performed a rearrangement of Fc N-glycans from a heterogeneous N-glycosylation pattern to homogeneous N-glycans using chemoenzymatic approaches with two types of endo-β-N-acetyl glucosaminidases (ENG'ases), one that works as a hydrolase to cleave all heterogeneous N-glycans, another that is used as a glycosynthase to generate homogeneous N-glycans. As starting materials, we used an anti-Her2 antibody produced in transgenic silkworm cocoon, which consists of non-fucosylated pauci-mannose type (Man2-3GlcNAc2), high-mannose type (Man4-9GlcNAc2), and complex type (Man3GlcNAc3-4) N-glycans. As a result of the cleavage of several ENG'ases (endoS, endoM, endoD, endoH, and endoLL), the heterogeneous glycans on antibodies were fully transformed into homogeneous-GlcNAc by a combination of endoS, endoD, and endoLL. Next, the desired N-glycans (M3; Man3GlcNAc1, G0; GlcNAc2Man3GlcNAc1, G2; Gal2GlcNAc2Man3GlcNAc1, A2; NeuAc2Gal2GlcNAc2Man3GlcNAc1) were transferred from the corresponding oxazolines to the GlcNAc residue on the intact anti-Her2 antibody with an ENG'ase mutant (endoS-D233Q), and the glycoengineered anti-Her2 antibody was obtained. The binding assay of anti-Her2 antibody with homogenous N-glycans with FcγRIIIa-V158 showed that the glycoform influenced the affinity for FcγRIIIa-V158. In addition, the ADCC assay for the glycoengineered anti-Her2 antibody (mAb-M3, mAb-G0, mAb-G2, and mAb-A2) was performed using SKBR-3 and BT-474 as target cells, and revealed that the glycoform influenced ADCC activity." @default.
• W1532698890 created "2016-06-24" @default.
• W1532698890 creator A5004270598 @default.
• W1532698890 creator A5005593466 @default.
• W1532698890 creator A5014802711 @default.
• W1532698890 creator A5023250885 @default.
• W1532698890 creator A5031525892 @default.
• W1532698890 creator A5031987607 @default.
• W1532698890 creator A5047201524 @default.
• W1532698890 creator A5058069479 @default.
• W1532698890 creator A5064170475 @default.
• W1532698890 creator A5064300186 @default.
• W1532698890 creator A5066571597 @default.
• W1532698890 creator A5070029030 @default.
• W1532698890 creator A5071914412 @default.
• W1532698890 creator A5075235588 @default.
• W1532698890 creator A5079099744 @default.
• W1532698890 date "2015-07-22" @default.
• W1532698890 modified "2023-10-18" @default.
• W1532698890 title "Glycoengineered Monoclonal Antibodies with Homogeneous Glycan (M3, G0, G2, and A2) Using a Chemoenzymatic Approach Have Different Affinities for FcγRIIIa and Variable Antibody-Dependent Cellular Cytotoxicity Activities" @default.
• W1532698890 cites W1485462441 @default.
• W1532698890 cites W1516116748 @default.
• W1532698890 cites W1518819083 @default.
• W1532698890 cites W1519001384 @default.
• W1532698890 cites W1529809181 @default.
• W1532698890 cites W1530276086 @default.
• W1532698890 cites W1582894915 @default.
• W1532698890 cites W1683971004 @default.
• W1532698890 cites W1825768998 @default.
• W1532698890 cites W1831036856 @default.
• W1532698890 cites W1969824500 @default.
• W1532698890 cites W1977255144 @default.
• W1532698890 cites W1979626824 @default.
• W1532698890 cites W1980003919 @default.
• W1532698890 cites W1984127523 @default.
• W1532698890 cites W1986262138 @default.
• W1532698890 cites W1989010611 @default.
• W1532698890 cites W1989418439 @default.
• W1532698890 cites W1990194904 @default.
• W1532698890 cites W1991465734 @default.
• W1532698890 cites W1997826619 @default.
• W1532698890 cites W1997937515 @default.
• W1532698890 cites W1998320362 @default.
• W1532698890 cites W1999989255 @default.
• W1532698890 cites W2000814379 @default.
• W1532698890 cites W2004919864 @default.
• W1532698890 cites W2011614021 @default.
• W1532698890 cites W2013264850 @default.
• W1532698890 cites W2016704104 @default.
• W1532698890 cites W2025966090 @default.
• W1532698890 cites W2030932286 @default.
• W1532698890 cites W2035433348 @default.
• W1532698890 cites W2035542094 @default.
• W1532698890 cites W2041630784 @default.
• W1532698890 cites W2043507805 @default.
• W1532698890 cites W2043920247 @default.
• W1532698890 cites W2045193638 @default.
• W1532698890 cites W2048313558 @default.
• W1532698890 cites W2048504768 @default.
• W1532698890 cites W2052491267 @default.
• W1532698890 cites W2053863608 @default.
• W1532698890 cites W2054270822 @default.
• W1532698890 cites W2057333689 @default.
• W1532698890 cites W2058232936 @default.
• W1532698890 cites W2063614195 @default.
• W1532698890 cites W2072100650 @default.
• W1532698890 cites W2075219903 @default.
• W1532698890 cites W2075305667 @default.
• W1532698890 cites W2075901113 @default.
• W1532698890 cites W2076751523 @default.
• W1532698890 cites W2076969588 @default.
• W1532698890 cites W2078162558 @default.
• W1532698890 cites W2081424327 @default.
• W1532698890 cites W2083982805 @default.
• W1532698890 cites W2084136419 @default.
• W1532698890 cites W2092532858 @default.
• W1532698890 cites W2106183724 @default.
• W1532698890 cites W2111421669 @default.
• W1532698890 cites W2115175735 @default.
• W1532698890 cites W2115525058 @default.
• W1532698890 cites W2115571948 @default.
• W1532698890 cites W2121033687 @default.
• W1532698890 cites W2121107218 @default.
• W1532698890 cites W2124847934 @default.
• W1532698890 cites W2125169711 @default.
• W1532698890 cites W2130350426 @default.
• W1532698890 cites W2137354517 @default.
• W1532698890 cites W2140035322 @default.
• W1532698890 cites W2150625529 @default.
• W1532698890 cites W2157003977 @default.
• W1532698890 cites W2169826182 @default.
• W1532698890 cites W2171275762 @default.
• W1532698890 cites W4293253343 @default.
• W1532698890 doi "https://doi.org/10.1371/journal.pone.0132848" @default.
• W1532698890 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4511734" @default.
• W1532698890 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26200113" @default.
• W1532698890 hasPublicationYear "2015" @default.
• W1532698890 type Work @default.

• next