FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1533018227> ?p ?o ?g. }

• W1533018227 endingPage "267" @default.
• W1533018227 startingPage "257" @default.
• W1533018227 abstract "The ortho-cleavage pathways of catechol and 3-chlorocatechol are central catabolic pathways of Pseudomonas putida that convert aromatic and chloroaromatic compounds to tricarboxylic acid (TCA)-cycle intermediates. They are encoded by the evolutionarily related catBCA and clcABD operons, respectively. Expression of the cat and clc operons requires the LysR-type transcriptional activators CatR and ClcR, and the inducer molecules cis,cis-muconate and 2-chloro-cis,cis-muconate. In addition to sequence similarities, CatR and ClcR share functional similarities which allow catR to complement clcR mutants. DNase-I footprinting, DNA bending and in vitro transcription analyses with RNA polymerase mutants indicate that CatR and ClcR activate transcription via a similar mechanism which involves interaction with the C-terminal domain of the alpha-subunit (alpha-CTD) of RNA polymerase. In vitro transcription assays with different regions of the clc promoter indicate that the ClcR dimer bound to the promoter proximal site (the activation binding site) interacts with the alpha-CTD. Gel shift assays and DNase-I footprinting have demonstrated that CatR occupies two adjacent sites proximal to the catBCA promoter in the presence of inducer and an additional binding site within the catB structural gene called the internal binding site (IBS). CatR binds the IBS with low intrinsic affinity that is increased by cooperativity in presence of the two promoter binding sites. Site-directed mutations in the IBS indicate a probable cis-acting repressor function for the IBS. The location of the IBS within the catB structural gene, the cooperativity observed in footprinting studies and phasing studies suggest that the IBS participates in the interaction of CatR with the upstream binding sites by looping out the intervening DNA. Although the core transcriptional activation mechanisms of CatR and ClcR have been conserved, nature has provided some flexibility to respond to different environmental signals in addition to the presence of inducer. Transcriptional fusion studies demonstrate that the expression from the clc promoter is repressed when the cells are grown on succinate, citrate or fumarate and that this repression is ClcR-dependent and occurs at the transcriptional level. The presence of these organic acids did not affect the expression from the cat promoter. In vitro transcription assays demonstrate that the TCA-cycle intermediate, fumarate, directly and specifically inhibits the formation of the clcA transcript. No such inhibition was observed when CatR was used as activator on either the cat or clc template. Since both the catechol and the chlorocatechol pathways feed into the TCA cycle, but only the chlorocatechol pathway is inhibited by fumarate, there is a subtle difference in the regulation of these two pathways where intracellular sensing of a TCA-cycle intermediate leads to a reduction of chloroaromatic degradation." @default.
• W1533018227 created "2016-06-24" @default.
• W1533018227 creator A5008527036 @default.
• W1533018227 creator A5036301234 @default.
• W1533018227 creator A5078466410 @default.
• W1533018227 date "1998-11-01" @default.
• W1533018227 modified "2023-10-18" @default.
• W1533018227 title "Transcriptional activation of the catechol and chlorocatechol operons: variations on a theme1Published in conjunction with A Wisconsin Gathering Honoring Waclaw Szybalski on the occasion of his 75th year and 20years of Editorship-in-Chief of Gene, 10–11 August, 1997, University of Wisconsin, Madison, WI, USA.1" @default.
• W1533018227 cites W1519327359 @default.
• W1533018227 cites W1551929316 @default.
• W1533018227 cites W1560866854 @default.
• W1533018227 cites W1586662827 @default.
• W1533018227 cites W1606753899 @default.
• W1533018227 cites W1636946600 @default.
• W1533018227 cites W1652118203 @default.
• W1533018227 cites W1687927156 @default.
• W1533018227 cites W1729074192 @default.
• W1533018227 cites W1736256052 @default.
• W1533018227 cites W1757552322 @default.
• W1533018227 cites W1786142408 @default.
• W1533018227 cites W1815658416 @default.
• W1533018227 cites W1877248188 @default.
• W1533018227 cites W1930218638 @default.
• W1533018227 cites W1935448673 @default.
• W1533018227 cites W1962362543 @default.
• W1533018227 cites W1963184160 @default.
• W1533018227 cites W1995036184 @default.
• W1533018227 cites W1995599083 @default.
• W1533018227 cites W2052334453 @default.
• W1533018227 cites W2080075765 @default.
• W1533018227 cites W2092685491 @default.
• W1533018227 cites W2097321270 @default.
• W1533018227 cites W2124976593 @default.
• W1533018227 cites W2125574936 @default.
• W1533018227 cites W2131062589 @default.
• W1533018227 cites W2131417039 @default.
• W1533018227 cites W2136276437 @default.
• W1533018227 cites W2143483463 @default.
• W1533018227 cites W2148987305 @default.
• W1533018227 cites W2156789779 @default.
• W1533018227 cites W2171655210 @default.
• W1533018227 cites W2174411387 @default.
• W1533018227 cites W2177832086 @default.
• W1533018227 cites W2179170929 @default.
• W1533018227 cites W93718454 @default.
• W1533018227 doi "https://doi.org/10.1016/s0378-1119(98)00366-7" @default.
• W1533018227 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9858745" @default.
• W1533018227 hasPublicationYear "1998" @default.
• W1533018227 type Work @default.
• W1533018227 sameAs 1533018227 @default.
• W1533018227 citedByCount "92" @default.
• W1533018227 countsByYear W15330182272013 @default.
• W1533018227 countsByYear W15330182272014 @default.
• W1533018227 countsByYear W15330182272015 @default.
• W1533018227 countsByYear W15330182272016 @default.
• W1533018227 countsByYear W15330182272017 @default.
• W1533018227 countsByYear W15330182272018 @default.
• W1533018227 countsByYear W15330182272019 @default.
• W1533018227 countsByYear W15330182272020 @default.
• W1533018227 countsByYear W15330182272021 @default.
• W1533018227 countsByYear W15330182272022 @default.
• W1533018227 countsByYear W15330182272023 @default.
• W1533018227 crossrefType "journal-article" @default.
• W1533018227 hasAuthorship W1533018227A5008527036 @default.
• W1533018227 hasAuthorship W1533018227A5036301234 @default.
• W1533018227 hasAuthorship W1533018227A5078466410 @default.
• W1533018227 hasConcept C101762097 @default.
• W1533018227 hasConcept C104317684 @default.
• W1533018227 hasConcept C107824862 @default.
• W1533018227 hasConcept C138885662 @default.
• W1533018227 hasConcept C143065580 @default.
• W1533018227 hasConcept C150194340 @default.
• W1533018227 hasConcept C153911025 @default.
• W1533018227 hasConcept C158448853 @default.
• W1533018227 hasConcept C166014724 @default.
• W1533018227 hasConcept C179926584 @default.
• W1533018227 hasConcept C203075996 @default.
• W1533018227 hasConcept C22427896 @default.
• W1533018227 hasConcept C27153228 @default.
• W1533018227 hasConcept C2776449523 @default.
• W1533018227 hasConcept C2778021871 @default.
• W1533018227 hasConcept C41895202 @default.
• W1533018227 hasConcept C55493867 @default.
• W1533018227 hasConcept C67705224 @default.
• W1533018227 hasConcept C86339819 @default.
• W1533018227 hasConcept C86803240 @default.
• W1533018227 hasConceptScore W1533018227C101762097 @default.
• W1533018227 hasConceptScore W1533018227C104317684 @default.
• W1533018227 hasConceptScore W1533018227C107824862 @default.
• W1533018227 hasConceptScore W1533018227C138885662 @default.
• W1533018227 hasConceptScore W1533018227C143065580 @default.
• W1533018227 hasConceptScore W1533018227C150194340 @default.
• W1533018227 hasConceptScore W1533018227C153911025 @default.
• W1533018227 hasConceptScore W1533018227C158448853 @default.
• W1533018227 hasConceptScore W1533018227C166014724 @default.
• W1533018227 hasConceptScore W1533018227C179926584 @default.
• W1533018227 hasConceptScore W1533018227C203075996 @default.
• W1533018227 hasConceptScore W1533018227C22427896 @default.

• next