Matches in SemOpenAlex for { <https://semopenalex.org/work/W153309364> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W153309364 endingPage "64" @default.
- W153309364 startingPage "2655" @default.
- W153309364 abstract "On reaching the surface position, corneal epithelial cells assemble a tight paracellular barrier rapidly. The purpose of this study was to identify at what maturation stage the cells acquire biochemical elements underpinning this ability.Rabbit corneas were subjected to a digitonin-exposure protocol that results in the devitalization and desquamation of the three outermost cell layers of the 5-6-layer thick epithelium and thus, exposes winglike cells to the tear surface. Corneas were then mounted in Ussing-type chambers to measure transepithelial resistance and solute fluxes.After the devitalization treatment transepithelial resistance remained near baseline for 2 hours and then rose to 8.24 +/- 3.12 K omega.cm2 (+/- SD, n = 15) during the next 8 hours. This increase was matched by increases in the resistance to the paracellular flow of mannitol. Transmission electron microscopy confirmed de novo formation of tight junctions over this time span in the new surface. The addition of transcription (actinomycin D; alpha-amanitin), translation (cycloheximide), core glycosylation (tunicamycin), and endoplasmic reticulum-to-Golgi traffic (brefeldin A) inhibitors immediately after the devitalization protocol prevented the transepithelial resistance raise. Introducing time delays between the cell devitalization and the addition of these biosynthesis inhibitors reduced their effect; a time delay of 90 minutes abolished the inhibition by alpha-amanitin.Devitalization of overlying cells induces the assembly of a tight paracellular barrier in the wing cells. The process requires and includes the synthesis of essential transcripts and proteins that were initially absent in these cells. In vivo, the synthesis of these elements must occur as cells reach the squamous stage of maturation." @default.
- W153309364 created "2016-06-24" @default.
- W153309364 creator A5032541628 @default.
- W153309364 creator A5072934731 @default.
- W153309364 date "1993-08-01" @default.
- W153309364 modified "2023-09-23" @default.
- W153309364 title "Ontogeny of corneal epithelial tight junctions: stratal locale of biosynthetic activities." @default.
- W153309364 cites W1633066991 @default.
- W153309364 cites W1882858899 @default.
- W153309364 cites W1974578205 @default.
- W153309364 cites W2015462966 @default.
- W153309364 cites W2025520796 @default.
- W153309364 cites W2032589500 @default.
- W153309364 cites W2045045562 @default.
- W153309364 cites W2068887966 @default.
- W153309364 cites W2074102584 @default.
- W153309364 cites W2075207396 @default.
- W153309364 cites W2087200521 @default.
- W153309364 cites W2093671559 @default.
- W153309364 cites W2107312415 @default.
- W153309364 cites W2119226652 @default.
- W153309364 cites W2171315853 @default.
- W153309364 cites W2305059849 @default.
- W153309364 cites W2410121141 @default.
- W153309364 cites W2411738355 @default.
- W153309364 cites W90304329 @default.
- W153309364 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8344789" @default.
- W153309364 hasPublicationYear "1993" @default.
- W153309364 type Work @default.
- W153309364 sameAs 153309364 @default.
- W153309364 citedByCount "6" @default.
- W153309364 crossrefType "journal-article" @default.
- W153309364 hasAuthorship W153309364A5032541628 @default.
- W153309364 hasAuthorship W153309364A5072934731 @default.
- W153309364 hasConcept C119062480 @default.
- W153309364 hasConcept C120882062 @default.
- W153309364 hasConcept C153911025 @default.
- W153309364 hasConcept C158617107 @default.
- W153309364 hasConcept C177779419 @default.
- W153309364 hasConcept C185592680 @default.
- W153309364 hasConcept C2778128677 @default.
- W153309364 hasConcept C2781373781 @default.
- W153309364 hasConcept C3675279 @default.
- W153309364 hasConcept C41625074 @default.
- W153309364 hasConcept C55493867 @default.
- W153309364 hasConcept C60903277 @default.
- W153309364 hasConcept C86803240 @default.
- W153309364 hasConcept C95444343 @default.
- W153309364 hasConceptScore W153309364C119062480 @default.
- W153309364 hasConceptScore W153309364C120882062 @default.
- W153309364 hasConceptScore W153309364C153911025 @default.
- W153309364 hasConceptScore W153309364C158617107 @default.
- W153309364 hasConceptScore W153309364C177779419 @default.
- W153309364 hasConceptScore W153309364C185592680 @default.
- W153309364 hasConceptScore W153309364C2778128677 @default.
- W153309364 hasConceptScore W153309364C2781373781 @default.
- W153309364 hasConceptScore W153309364C3675279 @default.
- W153309364 hasConceptScore W153309364C41625074 @default.
- W153309364 hasConceptScore W153309364C55493867 @default.
- W153309364 hasConceptScore W153309364C60903277 @default.
- W153309364 hasConceptScore W153309364C86803240 @default.
- W153309364 hasConceptScore W153309364C95444343 @default.
- W153309364 hasIssue "9" @default.
- W153309364 hasLocation W1533093641 @default.
- W153309364 hasOpenAccess W153309364 @default.
- W153309364 hasPrimaryLocation W1533093641 @default.
- W153309364 hasRelatedWork W1970071752 @default.
- W153309364 hasRelatedWork W1979368709 @default.
- W153309364 hasRelatedWork W1991882485 @default.
- W153309364 hasRelatedWork W2032925493 @default.
- W153309364 hasRelatedWork W2088821423 @default.
- W153309364 hasRelatedWork W2094656637 @default.
- W153309364 hasRelatedWork W2104518242 @default.
- W153309364 hasRelatedWork W2136504773 @default.
- W153309364 hasRelatedWork W4238377641 @default.
- W153309364 hasRelatedWork W4280529994 @default.
- W153309364 hasVolume "34" @default.
- W153309364 isParatext "false" @default.
- W153309364 isRetracted "false" @default.
- W153309364 magId "153309364" @default.
- W153309364 workType "article" @default.