Matches in SemOpenAlex for { <https://semopenalex.org/work/W1533135956> ?p ?o ?g. }
- W1533135956 abstract "Recombinant protein expression has become an invaluable tool for academic and biotechnological projects. With the use of high-throughput screening technologies for soluble protein production, uncountable target proteins were produced in a soluble and homogeneous state enabling the realization of further studies. Evaluation of hundreds conditions requires the use of high-throughput cloning and screening methods. Here we describe a new versatile vector suite dedicated to the expression improvement of recombinant proteins with solubility problems. This vector suite allows the parallel cloning of the same PCR product into the 12 different expression vectors evaluating protein expression under different promoter strength, different fusion tags as well as different solubility enhancer proteins. Additionally, we propose the use of a new fusion protein which appear to be a useful solubility enhancer. Overall in this work we propose an economic and useful vector suite to fast track the solubility of different recombinant proteins and we propose a new solubility enhancer protein that can be included in the evaluation of the expression of recombinant proteins that are insoluble in classical expression conditions." @default.
- W1533135956 created "2016-06-24" @default.
- W1533135956 creator A5025640506 @default.
- W1533135956 creator A5040047843 @default.
- W1533135956 creator A5053486944 @default.
- W1533135956 creator A5067059405 @default.
- W1533135956 creator A5079912873 @default.
- W1533135956 creator A5090923484 @default.
- W1533135956 date "2014-01-01" @default.
- W1533135956 modified "2023-09-25" @default.
- W1533135956 title "Generation of a vector suite for protein solubility screening" @default.
- W1533135956 cites W126946935 @default.
- W1533135956 cites W1519297291 @default.
- W1533135956 cites W1580227085 @default.
- W1533135956 cites W169428739 @default.
- W1533135956 cites W1795716959 @default.
- W1533135956 cites W1872665193 @default.
- W1533135956 cites W1965858950 @default.
- W1533135956 cites W1991821654 @default.
- W1533135956 cites W1992221689 @default.
- W1533135956 cites W1992238971 @default.
- W1533135956 cites W1997184203 @default.
- W1533135956 cites W2003944847 @default.
- W1533135956 cites W2004583149 @default.
- W1533135956 cites W2025391446 @default.
- W1533135956 cites W2025562774 @default.
- W1533135956 cites W2036826769 @default.
- W1533135956 cites W2047360542 @default.
- W1533135956 cites W2051095636 @default.
- W1533135956 cites W2056772703 @default.
- W1533135956 cites W2067779211 @default.
- W1533135956 cites W2069848278 @default.
- W1533135956 cites W2075222652 @default.
- W1533135956 cites W2094476648 @default.
- W1533135956 cites W2097152121 @default.
- W1533135956 cites W2099910773 @default.
- W1533135956 cites W2103747393 @default.
- W1533135956 cites W2122500226 @default.
- W1533135956 cites W2137606590 @default.
- W1533135956 cites W2161323536 @default.
- W1533135956 cites W2171377206 @default.
- W1533135956 cites W2409395644 @default.
- W1533135956 cites W2501915533 @default.
- W1533135956 cites W89691187 @default.
- W1533135956 cites W188593863 @default.
- W1533135956 doi "https://doi.org/10.3389/fmicb.2014.00067" @default.
- W1533135956 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3934309" @default.
- W1533135956 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24616717" @default.
- W1533135956 hasPublicationYear "2014" @default.
- W1533135956 type Work @default.
- W1533135956 sameAs 1533135956 @default.
- W1533135956 citedByCount "25" @default.
- W1533135956 countsByYear W15331359562014 @default.
- W1533135956 countsByYear W15331359562015 @default.
- W1533135956 countsByYear W15331359562017 @default.
- W1533135956 countsByYear W15331359562018 @default.
- W1533135956 countsByYear W15331359562019 @default.
- W1533135956 countsByYear W15331359562020 @default.
- W1533135956 countsByYear W15331359562021 @default.
- W1533135956 countsByYear W15331359562022 @default.
- W1533135956 countsByYear W15331359562023 @default.
- W1533135956 crossrefType "journal-article" @default.
- W1533135956 hasAuthorship W1533135956A5025640506 @default.
- W1533135956 hasAuthorship W1533135956A5040047843 @default.
- W1533135956 hasAuthorship W1533135956A5053486944 @default.
- W1533135956 hasAuthorship W1533135956A5067059405 @default.
- W1533135956 hasAuthorship W1533135956A5079912873 @default.
- W1533135956 hasAuthorship W1533135956A5090923484 @default.
- W1533135956 hasBestOaLocation W15331359561 @default.
- W1533135956 hasConcept C104317684 @default.
- W1533135956 hasConcept C111936080 @default.
- W1533135956 hasConcept C121050878 @default.
- W1533135956 hasConcept C123894998 @default.
- W1533135956 hasConcept C150194340 @default.
- W1533135956 hasConcept C155574463 @default.
- W1533135956 hasConcept C166957645 @default.
- W1533135956 hasConcept C178790620 @default.
- W1533135956 hasConcept C185592680 @default.
- W1533135956 hasConcept C199360897 @default.
- W1533135956 hasConcept C33161422 @default.
- W1533135956 hasConcept C40767141 @default.
- W1533135956 hasConcept C41008148 @default.
- W1533135956 hasConcept C55493867 @default.
- W1533135956 hasConcept C70721500 @default.
- W1533135956 hasConcept C79581498 @default.
- W1533135956 hasConcept C86803240 @default.
- W1533135956 hasConcept C92087593 @default.
- W1533135956 hasConcept C95457728 @default.
- W1533135956 hasConceptScore W1533135956C104317684 @default.
- W1533135956 hasConceptScore W1533135956C111936080 @default.
- W1533135956 hasConceptScore W1533135956C121050878 @default.
- W1533135956 hasConceptScore W1533135956C123894998 @default.
- W1533135956 hasConceptScore W1533135956C150194340 @default.
- W1533135956 hasConceptScore W1533135956C155574463 @default.
- W1533135956 hasConceptScore W1533135956C166957645 @default.
- W1533135956 hasConceptScore W1533135956C178790620 @default.
- W1533135956 hasConceptScore W1533135956C185592680 @default.
- W1533135956 hasConceptScore W1533135956C199360897 @default.
- W1533135956 hasConceptScore W1533135956C33161422 @default.
- W1533135956 hasConceptScore W1533135956C40767141 @default.