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- W1533158403 abstract "Publisher Summary The chapter presents a method developed to simultaneously analyze various isoforms of tocopherol and tocotrienol from biological samples using gradient elution chromatography and a coulometric electrode array detector. This detector is based on the use of multiple coulometric electrochemical sensors in series, maintained at different potentials. This allows for a combination of detector potentials, each optimal for a specific analyte. In summary, the HPLC-EC coulometric electrode array detection method described in the chapter sensitively detects isoforms of tocopherols and tocotrienol from biological samples. The detection limit for these compounds for this HPLC method is about 50 fmol. Using the extraction protocol, the recovery of tocopherol and tocotrienol from tissues was greater than 95%. Moreover, reproducibility of this HPLC method is excellent, with coefficient of variations ranging from 3 to 5%. The optimum potentials for the detection of various isoforms of tocopherol/tocotrienols are different. Therefore, maintaining an electrochemical sensor in a series at various potentials, each optimal for a given vitamin E isoform, will provide enhanced sensitivity in the detection of these compounds in biological samples, where the concentration of some of these vitamin E forms is very low." @default.
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- W1533158403 date "2002-01-01" @default.
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- W1533158403 title "[27] Simultaneous detection of tocopherols and tocotrienols in biological samples using HPLC-coulometric electrode array" @default.
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- W1533158403 doi "https://doi.org/10.1016/s0076-6879(02)52029-2" @default.
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