FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1534225882> ?p ?o ?g. }

• W1534225882 endingPage "1659" @default.
• W1534225882 startingPage "1650" @default.
• W1534225882 abstract "Abstract In contrast to all prokaryotic organisms previously studied, the fructose-P2 cleavage activity of crude Micrococcus aerogenes extracts was found to be insensitive to high levels of EDTA and o-phenanthroline. This observation suggested that M. aerogenes contained a Class I (Schiff base) fructose-P2 aldolase instead of the metallo (Class II)-aldolase typically found in other prokaryotes. The enzyme was purified about 1000-fold with 70% recovery. The purified aldolase, which appears essentially homogeneous by electrophoretic and gel filtration criteria, was completely active in the presence of 0.01 m EDTA and o-phenanthroline. In addition, direct Zn++ analysis on a metal-free preparation showed that a fully active enzyme sample contained only 0.01 g atom of Zn++ per mole. In contrast to Class II aldolases, the M. aerogenes enzyme lost activity on incubation with the substrate dihydroxyacetone-P in the presence of NaBH4. The degree of enzyme inactivation was directly correlated with the amount of [3H]dihydroxyacetone-P covalently bound to enzyme after treatment with NaBH4 and a stoichiometry of one dihydroxyacetone-P binding site per enzyme subunit was calculated. Identification of the substrate binding site as a lysyl residue by amino acid analysis establishes that this fructose-P2 aldolase is a Class I enzyme. The general catalytic features of the M. aerogenes enzyme resemble those of other Class I aldolases. (a) Its activity is not enhanced by the presence of K+. (b) It cleaves fructose-1-P at a significant rate (fructose-P2 to fructose-1-P activity ratio = 3.3). (c) It has a broad pH optima for fructose-P2 cleavage and a narrow optima for fructose-1-P cleavage; both pH profiles are similar to those of rabbit aldolases A and C. (d) The kinetic parameters Km and Vmax for the two substrates are very similar to those of rabbit aldolases. Km (fructose-P2) = 1 x 10-6 m; Km (fructose-1-P) = 1 x 10-2 m; Vmax (fructose-P2) = 8.2 units per mg; Vmax (fructose-1-P) = 2.4 units per mg. The enzyme, however, differs from other Class I aldolases in that its fructose-P2 cleavage activity is completely insensitive to digestion with carboxypeptidase A and its activity is unaffected by treatment with sulfhydryl reagents. M. aerogenes aldolase is distinct from all aldolases previously studied in that it is composed of a single polypeptide chain. The detection of only Class II aldolase activity in other micrococcus strains (Micrococcus lactilyticus, Micrococcus lysodeikticus, and Acidaminococcus fermentans) as well as all previous studies on the fructose-P2 aldolases of prokaryotic cells, suggest that the Class I aldolase described here has a very restricted distribution among the bacteria." @default.
• W1534225882 created "2016-06-24" @default.
• W1534225882 creator A5006763968 @default.
• W1534225882 creator A5036382303 @default.
• W1534225882 date "1973-03-01" @default.
• W1534225882 modified "2023-10-16" @default.
• W1534225882 title "A Class I (Schiff Base) Fructose Diphosphate Aldolase of Prokaryotic Origin" @default.
• W1534225882 cites W11583921 @default.
• W1534225882 cites W1481805127 @default.
• W1534225882 cites W1498581838 @default.
• W1534225882 cites W1522547987 @default.
• W1534225882 cites W1544291828 @default.
• W1534225882 cites W157927920 @default.
• W1534225882 cites W1580755471 @default.
• W1534225882 cites W1775749144 @default.
• W1534225882 cites W1962238503 @default.
• W1534225882 cites W1971035987 @default.
• W1534225882 cites W1973956782 @default.
• W1534225882 cites W1980732431 @default.
• W1534225882 cites W1994352879 @default.
• W1534225882 cites W1996595181 @default.
• W1534225882 cites W2002213258 @default.
• W1534225882 cites W2006370393 @default.
• W1534225882 cites W2017470279 @default.
• W1534225882 cites W2020901455 @default.
• W1534225882 cites W2021275615 @default.
• W1534225882 cites W2026912546 @default.
• W1534225882 cites W2038476778 @default.
• W1534225882 cites W2038615895 @default.
• W1534225882 cites W2039571045 @default.
• W1534225882 cites W2049800254 @default.
• W1534225882 cites W2050798469 @default.
• W1534225882 cites W2074364708 @default.
• W1534225882 cites W2077340384 @default.
• W1534225882 cites W2084978230 @default.
• W1534225882 cites W2093000132 @default.
• W1534225882 cites W2094299906 @default.
• W1534225882 cites W2094322231 @default.
• W1534225882 cites W2109870810 @default.
• W1534225882 cites W2111301002 @default.
• W1534225882 cites W2132068224 @default.
• W1534225882 cites W2258157615 @default.
• W1534225882 cites W2291856410 @default.
• W1534225882 cites W2335383741 @default.
• W1534225882 cites W2992124077 @default.
• W1534225882 cites W39635534 @default.
• W1534225882 cites W87122777 @default.
• W1534225882 doi "https://doi.org/10.1016/s0021-9258(19)44240-3" @default.
• W1534225882 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4348545" @default.
• W1534225882 hasPublicationYear "1973" @default.
• W1534225882 type Work @default.
• W1534225882 sameAs 1534225882 @default.
• W1534225882 citedByCount "59" @default.
• W1534225882 countsByYear W15342258822015 @default.
• W1534225882 crossrefType "journal-article" @default.
• W1534225882 hasAuthorship W1534225882A5006763968 @default.
• W1534225882 hasAuthorship W1534225882A5036382303 @default.
• W1534225882 hasBestOaLocation W15342258821 @default.
• W1534225882 hasConcept C134306372 @default.
• W1534225882 hasConcept C181199279 @default.
• W1534225882 hasConcept C184054246 @default.
• W1534225882 hasConcept C185592680 @default.
• W1534225882 hasConcept C2776428424 @default.
• W1534225882 hasConcept C2776970464 @default.
• W1534225882 hasConcept C28165981 @default.
• W1534225882 hasConcept C33923547 @default.
• W1534225882 hasConcept C3488265 @default.
• W1534225882 hasConcept C42058472 @default.
• W1534225882 hasConcept C55493867 @default.
• W1534225882 hasConcept C71240020 @default.
• W1534225882 hasConcept C86803240 @default.
• W1534225882 hasConceptScore W1534225882C134306372 @default.
• W1534225882 hasConceptScore W1534225882C181199279 @default.
• W1534225882 hasConceptScore W1534225882C184054246 @default.
• W1534225882 hasConceptScore W1534225882C185592680 @default.
• W1534225882 hasConceptScore W1534225882C2776428424 @default.
• W1534225882 hasConceptScore W1534225882C2776970464 @default.
• W1534225882 hasConceptScore W1534225882C28165981 @default.
• W1534225882 hasConceptScore W1534225882C33923547 @default.
• W1534225882 hasConceptScore W1534225882C3488265 @default.
• W1534225882 hasConceptScore W1534225882C42058472 @default.
• W1534225882 hasConceptScore W1534225882C55493867 @default.
• W1534225882 hasConceptScore W1534225882C71240020 @default.
• W1534225882 hasConceptScore W1534225882C86803240 @default.
• W1534225882 hasIssue "5" @default.
• W1534225882 hasLocation W15342258821 @default.
• W1534225882 hasOpenAccess W1534225882 @default.
• W1534225882 hasPrimaryLocation W15342258821 @default.
• W1534225882 hasRelatedWork W1595482599 @default.
• W1534225882 hasRelatedWork W1883877410 @default.
• W1534225882 hasRelatedWork W1965047979 @default.
• W1534225882 hasRelatedWork W1968993915 @default.
• W1534225882 hasRelatedWork W2008268302 @default.
• W1534225882 hasRelatedWork W2010795601 @default.
• W1534225882 hasRelatedWork W2024725061 @default.
• W1534225882 hasRelatedWork W2045534958 @default.
• W1534225882 hasRelatedWork W2065430999 @default.
• W1534225882 hasRelatedWork W2082347176 @default.

• next