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• W1534314296 abstract "Ternary complexes of vaccinia virus RNA polymerase containing 3'-OMeGMP-arrested transcripts were purified by native gel electrophoresis. These complexes resumed elongation in situ when gel slices were incubated with magnesium and NTPs. Elongation occurred in the absence of pyrophosphate, suggesting that the blocking 3'-OMeGMP residue was removed via a novel pathway. We show that purified elongation complexes contain an intrinsic nuclease activity that shortens nascent RNA from the 3'-end. RNA cleavage was absolutely dependent on a divalent cation and was stimulated by CTP. The initial 5' cleavage product remained associated with the ternary complex and could be elongated in the presence of NTPs. Multiple stepwise cleavages generated progressively shorter chains. Purified ternary complexes containing 3'-OH-terminated RNAs also displayed nuclease activity. Involvement of the vaccinia RNA polymerase subunit rpo30 in the transcript-shortening reaction is suggested based on sequence similarity of rpo30 to mammalian protein SII (TFIIS), an extrinsic transcription factor required for nascent RNA cleavage by RNA polymerase II (Reines, D. (1991) J. Biol. Chem. 267, 3795-3800)." @default.
• W1534314296 created "2016-06-24" @default.
• W1534314296 creator A5014112082 @default.
• W1534314296 creator A5035611130 @default.
• W1534314296 date "1993-01-01" @default.
• W1534314296 modified "2023-10-04" @default.
• W1534314296 title "Nascent RNA cleavage by purified ternary complexes of vaccinia RNA polymerase." @default.
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• W1534314296 doi "https://doi.org/10.1016/s0021-9258(18)53977-6" @default.
• W1534314296 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7678416" @default.
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