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- W1534622291 abstract "Whole-cell preparations of Clostridium thermoaceticum were exposed to a short pulse of 14 CO 2 under conditions in which double-labeled acetate was synthesized. Radioactive methyltetrahydrofolate monoglutamate, diglutamate, and triglutamates were isolated from extracts of the cells. The radioactivity was found to be exclusively in the five methyl position. The specific activities of the methyltetrahydrofolate derivatives were very high and were in accord with the proposal that methyltetrahydrofolates are the precursors of the methyl of acetate. A new method of separation of folates employing QAE-Sephadex chromatography and a linear gradient with triethylammonium bicarbonate is presented which completely resolves the common folate monoglutamates and, upon freeze-drying, yields salt-free preparations." @default.
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- W1534622291 date "1971-11-01" @default.
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- W1534622291 title "Total Synthesis of Acetate from CO <sub>2</sub> : Methyltetrahydrofolate, an Intermediate, and a Procedure for Separation of the Folates" @default.
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- W1534622291 doi "https://doi.org/10.1128/jb.108.2.770-776.1971" @default.
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