FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1534833050> ?p ?o ?g. }

• W1534833050 endingPage "1940" @default.
• W1534833050 startingPage "1931" @default.
• W1534833050 abstract "The essential herpes simplex virus type 1 (HSV-1) immediate-early IE63 (ICP27) is pleiotropic in function, promoting the switch from the early to late phase of virus gene expression, and has effects on the posttranscriptional processes of mRNA splicing and 3' processing. We have investigated the role of IE63 in the regulation of viral mRNA 3' processing and of late gene expression. Our in vitro 3' processing studies demonstrated that HSV-1 infection induces an activity, which requires IE63 gene expression, responsible for an observed increase in 3' processing of selected HSV-1 poly(A) sites. Processing efficiencies at the poly(A) sites of two late genes, UL38 and UL44, shown to be inherently weak processing sites, were increased by the IE63-induced activity. In contrast, 3' processing at the poly(A) sites of selected immediate-early and early genes, stronger processing sites, was unaffected by IE63 expression. UV cross-linking experiments demonstrated that HSV infection caused enhanced binding of protein factors, including the 64-kDa component of cleavage stimulation factor (CstF), to poly(A) site RNAs from virus genes of all temporal classes and that this enhanced binding required expression of IE63. By immunofluorescence, the homogeneous pattern of the 64-kDa CstF protein distribution became slightly clumped with infection, whereas the splicing small nuclear ribonucleoprotein particles were recognized into a highly punctate distribution away from the sites of virus transcription. This effect could create an increase in the relative concentration of 3' processing factors available to pre-mRNAs. Western blot (immunoblot) analysis showed that IE63 was required for expression of several true late genes and for the efficient and timely expression of the UL29 and UL42 early genes, integral components on the viral DNA synthesis machinery. Our data are consistent with two effects of IE63 on late gene regulation: firstly, a stimulation of pre-mRNA 3' processing and, secondly, as a requirement for expression of functions necessary for viral DNA synthesis." @default.
• W1534833050 created "2016-06-24" @default.
• W1534833050 creator A5001047119 @default.
• W1534833050 creator A5002496378 @default.
• W1534833050 creator A5014990149 @default.
• W1534833050 creator A5079233773 @default.
• W1534833050 date "1996-03-01" @default.
• W1534833050 modified "2023-09-27" @default.
• W1534833050 title "Regulation of herpes simplex virus poly (A) site usage and the action of immediate-early protein IE63 in the early-late switch" @default.
• W1534833050 cites W117601042 @default.
• W1534833050 cites W1488258440 @default.
• W1534833050 cites W1489207129 @default.
• W1534833050 cites W1507725858 @default.
• W1534833050 cites W1559441251 @default.
• W1534833050 cites W1573214389 @default.
• W1534833050 cites W1583466448 @default.
• W1534833050 cites W1588168133 @default.
• W1534833050 cites W1628312488 @default.
• W1534833050 cites W1733825645 @default.
• W1534833050 cites W1738338042 @default.
• W1534833050 cites W1764884856 @default.
• W1534833050 cites W1791309331 @default.
• W1534833050 cites W1875884436 @default.
• W1534833050 cites W1894393726 @default.
• W1534833050 cites W1895304651 @default.
• W1534833050 cites W1900646268 @default.
• W1534833050 cites W1919926061 @default.
• W1534833050 cites W1927078541 @default.
• W1534833050 cites W1928488550 @default.
• W1534833050 cites W1940400153 @default.
• W1534833050 cites W1954273049 @default.
• W1534833050 cites W1956393694 @default.
• W1534833050 cites W1964340993 @default.
• W1534833050 cites W1973014323 @default.
• W1534833050 cites W1974007922 @default.
• W1534833050 cites W1981599663 @default.
• W1534833050 cites W1995556647 @default.
• W1534833050 cites W2013076780 @default.
• W1534833050 cites W2032033331 @default.
• W1534833050 cites W2036460837 @default.
• W1534833050 cites W2047854594 @default.
• W1534833050 cites W2048167369 @default.
• W1534833050 cites W2056650797 @default.
• W1534833050 cites W2061163695 @default.
• W1534833050 cites W2083699794 @default.
• W1534833050 cites W2088291148 @default.
• W1534833050 cites W2116741012 @default.
• W1534833050 cites W2118300475 @default.
• W1534833050 cites W2140894105 @default.
• W1534833050 cites W2142280333 @default.
• W1534833050 cites W2147990979 @default.
• W1534833050 cites W2151018530 @default.
• W1534833050 cites W2156102853 @default.
• W1534833050 cites W2166195788 @default.
• W1534833050 cites W2167414261 @default.
• W1534833050 cites W2175025904 @default.
• W1534833050 cites W233498171 @default.
• W1534833050 cites W45102178 @default.
• W1534833050 cites W52468748 @default.
• W1534833050 cites W55933576 @default.
• W1534833050 doi "https://doi.org/10.1128/jvi.70.3.1931-1940.1996" @default.
• W1534833050 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/190022" @default.
• W1534833050 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8627719" @default.
• W1534833050 hasPublicationYear "1996" @default.
• W1534833050 type Work @default.
• W1534833050 sameAs 1534833050 @default.
• W1534833050 citedByCount "114" @default.
• W1534833050 countsByYear W15348330502012 @default.
• W1534833050 countsByYear W15348330502013 @default.
• W1534833050 countsByYear W15348330502014 @default.
• W1534833050 countsByYear W15348330502015 @default.
• W1534833050 countsByYear W15348330502016 @default.
• W1534833050 countsByYear W15348330502017 @default.
• W1534833050 countsByYear W15348330502018 @default.
• W1534833050 countsByYear W15348330502019 @default.
• W1534833050 countsByYear W15348330502020 @default.
• W1534833050 countsByYear W15348330502021 @default.
• W1534833050 crossrefType "journal-article" @default.
• W1534833050 hasAuthorship W1534833050A5001047119 @default.
• W1534833050 hasAuthorship W1534833050A5002496378 @default.
• W1534833050 hasAuthorship W1534833050A5014990149 @default.
• W1534833050 hasAuthorship W1534833050A5079233773 @default.
• W1534833050 hasBestOaLocation W15348330501 @default.
• W1534833050 hasConcept C102063271 @default.
• W1534833050 hasConcept C104317684 @default.
• W1534833050 hasConcept C105580179 @default.
• W1534833050 hasConcept C138885662 @default.
• W1534833050 hasConcept C142575336 @default.
• W1534833050 hasConcept C150194340 @default.
• W1534833050 hasConcept C153911025 @default.
• W1534833050 hasConcept C159047783 @default.
• W1534833050 hasConcept C179926584 @default.
• W1534833050 hasConcept C2522874641 @default.
• W1534833050 hasConcept C2781196997 @default.
• W1534833050 hasConcept C2908584439 @default.
• W1534833050 hasConcept C41895202 @default.
• W1534833050 hasConcept C54355233 @default.
• W1534833050 hasConcept C54458228 @default.

• next