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• W1534865720 abstract "Article1 June 1986free access Overdrive, a T-DNA transmission enhancer on the A. tumefaciens tumour-inducing plasmid Ernest G. Peralta Ernest G. Peralta Institute for Molecular Biology, Department of Biology, Indiana University, Bloomington, IN 47405, USA Present address: Genentech Inc., 460 Point San Bruno Boulevard, South San Francisco, CA 94080, USA Search for more papers by this author Renate Hellmiss Renate Hellmiss Institute for Molecular Biology, Department of Biology, Indiana University, Bloomington, IN 47405, USA Search for more papers by this author Walt Ream Walt Ream Institute for Molecular Biology, Department of Biology, Indiana University, Bloomington, IN 47405, USA Search for more papers by this author Ernest G. Peralta Ernest G. Peralta Institute for Molecular Biology, Department of Biology, Indiana University, Bloomington, IN 47405, USA Present address: Genentech Inc., 460 Point San Bruno Boulevard, South San Francisco, CA 94080, USA Search for more papers by this author Renate Hellmiss Renate Hellmiss Institute for Molecular Biology, Department of Biology, Indiana University, Bloomington, IN 47405, USA Search for more papers by this author Walt Ream Walt Ream Institute for Molecular Biology, Department of Biology, Indiana University, Bloomington, IN 47405, USA Search for more papers by this author Author Information Ernest G. Peralta1, Renate Hellmiss1 and Walt Ream1 1Institute for Molecular Biology, Department of Biology, Indiana University, Bloomington, IN 47405, USA The EMBO Journal (1986)5:1137-1142https://doi.org/10.1002/j.1460-2075.1986.tb04338.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info During crown gall tumorigenesis a specific segment of the Agrobacterium tumefaciens tumour-inducing (Ti) plasmid, the T-DNA, integrates into plant nuclear DNA. Similar 23-bp direct repeats at each end of the T region signal T-DNA borders, and T-DNA transmission (transfer and integration) requires the right-hand direct repeat. A chemically synthesized right border repeat in its wild-type orientation promotes T-DNA transmission at a low frequency; Ti plasmid sequences which normally flank the right repeat greatly stimulate the process. To identify flanking sequences required for full right border activity, we tested the activity of a border repeat surrounded by different amounts of normal flanking sequences. Efficient T-DNA transmission required a conserved sequence (5′ TAAPuTPy-CTGTPuT-TGTTTGTTTG 3′) which lies to the right of the two known right border repeats. In either orientation, a synthetic oligonucleotide containing this conserved sequence greatly stimulated the activity of a right border repeat, and a deletion removing 15 bp from the right end of this sequence destroyed its stimulatory effect. Thus, wild-type T-DNA transmission required both the 23-bp right border repeat and a conserved flanking sequence which we call overdrive. Previous ArticleNext Article Volume 5Issue 61 June 1986In this issue RelatedDetailsLoading ..." @default.
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• W1534865720 title "<i>Overdrive</i> , a T-DNA transmission enhancer on the <i>A. tumefaciens</i> tumour-inducing plasmid" @default.
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• W1534865720 doi "https://doi.org/10.1002/j.1460-2075.1986.tb04338.x" @default.
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