Matches in SemOpenAlex for { <https://semopenalex.org/work/W1535163517> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W1535163517 endingPage "8320" @default.
- W1535163517 startingPage "8315" @default.
- W1535163517 abstract "An acidic protein, extractable in neutral salt solutions from rat skin, was markedly enriched when precipitated by dialysis against 0.5 M acetic acid. After dissolving the precipitate in 0.5 M Tris-HCl buffer, pH 8.0, the protein was disaggregated by the addition of the nonionic detergent Triton X-100 and purified by chromatography on Sephadex G-100 and DEAE-Sephadex A-50 columns. The protein isolated under nondenaturing conditions appeared to be essentially homogeneous by its migration as a single band on (a) cellulose acetate membrane electrophoresis at pH 8.6; (B) 4% and 7.5% polyacrylamide gel electrophoresis at ph 8.9; (C) sodium dodecyl sulfate (10%) polyacrylamide gel electrophoresis at pH 7.0; and by (d) its complete freedom from collagen, the major contaminating protein. The molecular weight of the protein was determined as 76,000 +/- 2,000 from its electrophoretic mobility in sodium dodecyl sulfate polyacrylamide gels and 75,000 from its elution volume in Sephadex G-100 columns. Reduction and alkylation of the protein failed to generate smaller subunits. The amino acid composition of the protein showed that it was relatively rich in glutamic and aspartic acids, which together comprised 25% of its total residues. Hydrophobic amino acids like phenylalanine, leucine, isoleucine, valine, methionine, alanine, proline, and cystine accounted for about 34% of the total residues in the protein. No free NH2-terminal amino acid could be detected in the purified protein by the dansylation method. Each mole of protein contained 11 mol of phosphate. Triton X-100 was necessary for achieving nondestructive disaggregation of the acidic protein. Each mole of protein bound about 3200 mol of Triton X-100 or 10 mol of Congo red. While the detergent binding could be reversed by dialysis, Congo red formed a stable complex with the protein." @default.
- W1535163517 created "2016-06-24" @default.
- W1535163517 creator A5081840318 @default.
- W1535163517 creator A5091350437 @default.
- W1535163517 date "1975-11-01" @default.
- W1535163517 modified "2023-09-26" @default.
- W1535163517 title "Purification and properties of an acidic protein from rat skin." @default.
- W1535163517 cites W1139343036 @default.
- W1535163517 cites W1556268921 @default.
- W1535163517 cites W1563440992 @default.
- W1535163517 cites W1594223211 @default.
- W1535163517 cites W1599125820 @default.
- W1535163517 cites W1625660792 @default.
- W1535163517 cites W1775749144 @default.
- W1535163517 cites W1964261202 @default.
- W1535163517 cites W1966798326 @default.
- W1535163517 cites W1967979243 @default.
- W1535163517 cites W1968931474 @default.
- W1535163517 cites W1984501109 @default.
- W1535163517 cites W1985342306 @default.
- W1535163517 cites W1987484067 @default.
- W1535163517 cites W1988478867 @default.
- W1535163517 cites W1989675515 @default.
- W1535163517 cites W1992964172 @default.
- W1535163517 cites W1995243047 @default.
- W1535163517 cites W2003725197 @default.
- W1535163517 cites W2010320660 @default.
- W1535163517 cites W2010940575 @default.
- W1535163517 cites W2017059567 @default.
- W1535163517 cites W2019237858 @default.
- W1535163517 cites W2023712946 @default.
- W1535163517 cites W2027880243 @default.
- W1535163517 cites W2061477796 @default.
- W1535163517 cites W2065912276 @default.
- W1535163517 cites W2080109475 @default.
- W1535163517 cites W2085163144 @default.
- W1535163517 cites W2092153634 @default.
- W1535163517 cites W2093266001 @default.
- W1535163517 cites W2122641627 @default.
- W1535163517 cites W2153719065 @default.
- W1535163517 cites W2170550941 @default.
- W1535163517 cites W2315219698 @default.
- W1535163517 cites W2336752846 @default.
- W1535163517 cites W2394968116 @default.
- W1535163517 cites W48279760 @default.
- W1535163517 doi "https://doi.org/10.1016/s0021-9258(19)40761-8" @default.
- W1535163517 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/811654" @default.
- W1535163517 hasPublicationYear "1975" @default.
- W1535163517 type Work @default.
- W1535163517 sameAs 1535163517 @default.
- W1535163517 citedByCount "5" @default.
- W1535163517 crossrefType "journal-article" @default.
- W1535163517 hasAuthorship W1535163517A5081840318 @default.
- W1535163517 hasAuthorship W1535163517A5091350437 @default.
- W1535163517 hasBestOaLocation W15351635171 @default.
- W1535163517 hasConcept C185592680 @default.
- W1535163517 hasConcept C43617362 @default.
- W1535163517 hasConcept C55493867 @default.
- W1535163517 hasConceptScore W1535163517C185592680 @default.
- W1535163517 hasConceptScore W1535163517C43617362 @default.
- W1535163517 hasConceptScore W1535163517C55493867 @default.
- W1535163517 hasIssue "21" @default.
- W1535163517 hasLocation W15351635171 @default.
- W1535163517 hasOpenAccess W1535163517 @default.
- W1535163517 hasPrimaryLocation W15351635171 @default.
- W1535163517 hasRelatedWork W1531601525 @default.
- W1535163517 hasRelatedWork W1990781990 @default.
- W1535163517 hasRelatedWork W2033045522 @default.
- W1535163517 hasRelatedWork W2314643286 @default.
- W1535163517 hasRelatedWork W2344122601 @default.
- W1535163517 hasRelatedWork W2607424097 @default.
- W1535163517 hasRelatedWork W2748952813 @default.
- W1535163517 hasRelatedWork W2899084033 @default.
- W1535163517 hasRelatedWork W2948807893 @default.
- W1535163517 hasRelatedWork W2778153218 @default.
- W1535163517 hasVolume "250" @default.
- W1535163517 isParatext "false" @default.
- W1535163517 isRetracted "false" @default.
- W1535163517 magId "1535163517" @default.
- W1535163517 workType "article" @default.