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- W1535164477 abstract "With a view to identifying the molecular subtype of the circulating dengue virus responsible for a major outbreak of dengue fever (DF) / dengue haemorrhagic fever (DHF) in and around Delhi during the postmonsoon period in 2003, 32 serum samples were collected from clinically suspected cases. These were subjected to reverse transcription/polymerase chain reaction (RT/PCR) for amplification of 511 bp C PreM gene region of the dengue virus. Seven specimens, yielding a satisfactory quantum of viral RNA, were subsequently processed for automated nucleotide sequencing. Five of the seven analysed isolates showed close DNA sequence homology with Guate96-98 strains of DEN-3 virus, whereas two turned out to be genotype IV of DEN-2. Earlier, DEN-2 (genotype IV) had been identified as the etiological agent during a major DF/DHF outbreak in Delhi in 1996 and also in 2000. Though DEN-2 continues to prevail, DEN-3, having a close sequence homology with Guate96-98 strains, seems to have entered India for the first time in late 2003, resulting in a major DF/DHF outbreak. How the Guate96-98 strain of DEN -3 entered India remains to be linked epidemiologically." @default.
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- W1535164477 date "2004-01-01" @default.
- W1535164477 modified "2023-10-06" @default.
- W1535164477 title "Unusual Emergence of Guate98-like Molecular Subtype of DEN-3 during 2003 Dengue Outbreak in Delhi" @default.
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