FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1535270542> ?p ?o ?g. }

• W1535270542 endingPage "957" @default.
• W1535270542 startingPage "946" @default.
• W1535270542 abstract "Our previous studies have identified a role for annexin 1 (ANXA1), a protein produced by the pituitary folliculostellate cells, as a paracrine/juxtacrine mediator of the acute regulatory effects of glucocorticoids on the release of adrenocorticotropic hormone and other pituitary hormones. In the present study, we focused on the secretion of thyroid stimulating hormone (TSH) and luteinizing hormone (LH) and used a battery of ANXA1-derived peptides to identify the key domains in the ANXA1 molecule that are critical to the inhibition of peptide release. In addition, as ANXA1 is a substrate for protein kinase C (PKC) and tyrosine kinase, we examined the roles of these kinases in the manifestation of the ANXA1-dependent inhibitory actions of dexamethasone on TSH and LH release. Dexamethasone suppressed the forskolin-induced release of TSH and LH from rat anterior pituitary tissue in vitro. Its effects were mimicked by human recombinant ANXA1 (hrANXA1) and a truncated protein, ANXA11-188. ANXA1Ac2−26, also suppressed stimulated peptide release but it lacked both the potency and the efficacy of the parent protein. Shorter N-terminal ANXA1 sequences were without effect. The PKC inhibitor PKC19-36 abolished the inhibitory actions of dexamethasone on the forskolin-evoked release of TSH and LH; it also attenuated the inhibitory actions of ANXA1Ac2−26. Similar effects were produced by annexin 5 (ANXA5) which sequesters PKC in other systems. By contrast, the tyrosine kinase inhibitors, p60v-src (137–157) and genistein, had no effect on the secretion of TSH or LH alone or in the presence of forskolin and/or dexamethasone. Dexamethasone caused the translocation of a tyrosine-phosphorylated species of ANXA1 to the surface of pituitary cells. The total amount of ANXA1 exported from the cells in response to the steroid was unaffected by tyrosine kinase blockade. However, the degree of tyrosine-phosphorylation of the exported protein was markedly reduced by genistein. These results suggest that (i) the ANXA1-dependent inhibitory actions of dexamethasone on the release of TSH and LH require PKC and sequences in the N-terminal domain of ANXA1, but are independent of tyrosine kinase, and (ii) while dexamethasone induces the cellular exportation of a tyrosine-phosphorylated species of ANXA1, tyrosine phosphorylation per se is not critical to the steroid-induced passage of ANXA1 across the membrane." @default.
• W1535270542 created "2016-06-24" @default.
• W1535270542 creator A5003096777 @default.
• W1535270542 creator A5030517920 @default.
• W1535270542 creator A5030974804 @default.
• W1535270542 creator A5055174868 @default.
• W1535270542 creator A5060397110 @default.
• W1535270542 creator A5085315391 @default.
• W1535270542 date "2003-09-10" @default.
• W1535270542 modified "2023-09-25" @default.
• W1535270542 title "Kinase-Dependent Regulation of the Secretion of Thyrotrophin and Luteinizing Hormone By Glucocorticoids and Annexin 1 Peptides" @default.
• W1535270542 cites W1510619282 @default.
• W1535270542 cites W1510923466 @default.
• W1535270542 cites W1558378327 @default.
• W1535270542 cites W1582315378 @default.
• W1535270542 cites W1586330982 @default.
• W1535270542 cites W1642525803 @default.
• W1535270542 cites W1656644300 @default.
• W1535270542 cites W1714325720 @default.
• W1535270542 cites W1939115083 @default.
• W1535270542 cites W1970236236 @default.
• W1535270542 cites W1971639265 @default.
• W1535270542 cites W1974392294 @default.
• W1535270542 cites W1976188281 @default.
• W1535270542 cites W1979917335 @default.
• W1535270542 cites W1980610216 @default.
• W1535270542 cites W1983268250 @default.
• W1535270542 cites W1983533888 @default.
• W1535270542 cites W1986313866 @default.
• W1535270542 cites W1986482736 @default.
• W1535270542 cites W1987074935 @default.
• W1535270542 cites W1987250516 @default.
• W1535270542 cites W1988037602 @default.
• W1535270542 cites W1988330974 @default.
• W1535270542 cites W1990623931 @default.
• W1535270542 cites W1993913708 @default.
• W1535270542 cites W1996963674 @default.
• W1535270542 cites W1999251037 @default.
• W1535270542 cites W2000294771 @default.
• W1535270542 cites W2000889431 @default.
• W1535270542 cites W2004317005 @default.
• W1535270542 cites W2008672256 @default.
• W1535270542 cites W2017671033 @default.
• W1535270542 cites W2020995181 @default.
• W1535270542 cites W2029141549 @default.
• W1535270542 cites W2033212588 @default.
• W1535270542 cites W2034547873 @default.
• W1535270542 cites W2039885547 @default.
• W1535270542 cites W2041107578 @default.
• W1535270542 cites W2041557269 @default.
• W1535270542 cites W2043731021 @default.
• W1535270542 cites W2044297698 @default.
• W1535270542 cites W2052500741 @default.
• W1535270542 cites W2055881665 @default.
• W1535270542 cites W2058769642 @default.
• W1535270542 cites W2069309653 @default.
• W1535270542 cites W2069585979 @default.
• W1535270542 cites W2076179697 @default.
• W1535270542 cites W2080257815 @default.
• W1535270542 cites W2088019196 @default.
• W1535270542 cites W2089939142 @default.
• W1535270542 cites W2090755922 @default.
• W1535270542 cites W2090992599 @default.
• W1535270542 cites W2091673674 @default.
• W1535270542 cites W2097890791 @default.
• W1535270542 cites W2103994273 @default.
• W1535270542 cites W2136091506 @default.
• W1535270542 cites W2144839060 @default.
• W1535270542 cites W2148321746 @default.
• W1535270542 cites W2167861311 @default.
• W1535270542 cites W2170252001 @default.
• W1535270542 cites W2412490099 @default.
• W1535270542 cites W2418730364 @default.
• W1535270542 cites W4293247451 @default.
• W1535270542 doi "https://doi.org/10.1046/j.1365-2826.2003.01081.x" @default.
• W1535270542 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12969239" @default.
• W1535270542 hasPublicationYear "2003" @default.
• W1535270542 type Work @default.
• W1535270542 sameAs 1535270542 @default.
• W1535270542 citedByCount "39" @default.
• W1535270542 countsByYear W15352705422012 @default.
• W1535270542 countsByYear W15352705422013 @default.
• W1535270542 countsByYear W15352705422014 @default.
• W1535270542 countsByYear W15352705422015 @default.
• W1535270542 countsByYear W15352705422017 @default.
• W1535270542 countsByYear W15352705422018 @default.
• W1535270542 countsByYear W15352705422019 @default.
• W1535270542 countsByYear W15352705422020 @default.
• W1535270542 countsByYear W15352705422021 @default.
• W1535270542 countsByYear W15352705422022 @default.
• W1535270542 countsByYear W15352705422023 @default.
• W1535270542 crossrefType "journal-article" @default.
• W1535270542 hasAuthorship W1535270542A5003096777 @default.
• W1535270542 hasAuthorship W1535270542A5030517920 @default.
• W1535270542 hasAuthorship W1535270542A5030974804 @default.
• W1535270542 hasAuthorship W1535270542A5055174868 @default.
• W1535270542 hasAuthorship W1535270542A5060397110 @default.
• W1535270542 hasAuthorship W1535270542A5085315391 @default.

• next