Matches in SemOpenAlex for { <https://semopenalex.org/work/W1535367923> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W1535367923 endingPage "9451" @default.
- W1535367923 startingPage "9443" @default.
- W1535367923 abstract "Abstract The multifunctional protein uridine 5'-monophosphate (UMP) synthase catalyzes the final two reactions of the de novo biosynthesis of UMP in mammalian cells by the sequential action of orotate phosphoribosyltransferase (EC 2.4.2.10) and orotidine 5'-monophosphate (OMP) decarboxylase (EC 4.1.1.23). This protein is composed of one or two identical subunits; the monomer weighs of 51,500 daltons. UMP synthase from mouse Ehrlich ascites cells can exist as three distinct species as determined by sucrose density gradient centrifugation: a 3.6 S monomer, a 5.1 S dimer, and a 5.6 S conformationally altered dimer. Limited digestion of each of these three species with trypsin produced a 28,500-dalton peptide that was relatively resistant to further proteolysis. The peptide appears to be one of the two enzyme domains of UMP synthase for it retained only OMP decarboxylase activity. Similar results were obtained when UMP synthase was digested with elastase. OMP decarboxylase activity was less stable for the domain than for UMP synthase; the domain can rapidly lose activity upon storage or upon dilution. The size of the mammalian OMP decarboxylase domain is similar to that of yeast OMP decarboxylase. If the polypeptides which are cleaved from UMP synthase by trypsin are derived exclusively from either the amino or the carboxyl end of UMP synthase, then the size of a fragment possessing the orotate phosphoribosyltransferase domain could be as large as 23,000 daltons which is similar in size to the orotate phosphoribosyltransferase of yeast and of Escherichia coli." @default.
- W1535367923 created "2016-06-24" @default.
- W1535367923 creator A5006295693 @default.
- W1535367923 creator A5029417472 @default.
- W1535367923 date "1985-08-01" @default.
- W1535367923 modified "2023-09-29" @default.
- W1535367923 title "Isolation and characterization of the orotidine 5‘-monophosphate decarboxylase domain of the multifunctional protein uridine 5‘-monophosphate synthase." @default.
- W1535367923 cites W1501442122 @default.
- W1535367923 cites W1501912047 @default.
- W1535367923 cites W1508501487 @default.
- W1535367923 cites W1514925242 @default.
- W1535367923 cites W1526914386 @default.
- W1535367923 cites W1548719481 @default.
- W1535367923 cites W1581152304 @default.
- W1535367923 cites W1654031744 @default.
- W1535367923 cites W1656313990 @default.
- W1535367923 cites W1775749144 @default.
- W1535367923 cites W1966497279 @default.
- W1535367923 cites W1987508231 @default.
- W1535367923 cites W1991785865 @default.
- W1535367923 cites W1998343637 @default.
- W1535367923 cites W2006170518 @default.
- W1535367923 cites W2022961230 @default.
- W1535367923 cites W2037354287 @default.
- W1535367923 cites W2038362219 @default.
- W1535367923 cites W2042158789 @default.
- W1535367923 cites W2045251366 @default.
- W1535367923 cites W2096743624 @default.
- W1535367923 cites W2100837269 @default.
- W1535367923 cites W2144043129 @default.
- W1535367923 cites W2150769784 @default.
- W1535367923 cites W4249794067 @default.
- W1535367923 cites W4293247451 @default.
- W1535367923 cites W6261644 @default.
- W1535367923 cites W66018953 @default.
- W1535367923 cites W960483261 @default.
- W1535367923 doi "https://doi.org/10.1016/s0021-9258(17)39386-9" @default.
- W1535367923 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3839509" @default.
- W1535367923 hasPublicationYear "1985" @default.
- W1535367923 type Work @default.
- W1535367923 sameAs 1535367923 @default.
- W1535367923 citedByCount "34" @default.
- W1535367923 countsByYear W15353679232015 @default.
- W1535367923 countsByYear W15353679232017 @default.
- W1535367923 crossrefType "journal-article" @default.
- W1535367923 hasAuthorship W1535367923A5006295693 @default.
- W1535367923 hasAuthorship W1535367923A5029417472 @default.
- W1535367923 hasBestOaLocation W15353679231 @default.
- W1535367923 hasConcept C104317684 @default.
- W1535367923 hasConcept C112243037 @default.
- W1535367923 hasConcept C181199279 @default.
- W1535367923 hasConcept C185592680 @default.
- W1535367923 hasConcept C2775941552 @default.
- W1535367923 hasConcept C2779876172 @default.
- W1535367923 hasConcept C2781259782 @default.
- W1535367923 hasConcept C55493867 @default.
- W1535367923 hasConcept C60644358 @default.
- W1535367923 hasConcept C67705224 @default.
- W1535367923 hasConcept C86803240 @default.
- W1535367923 hasConceptScore W1535367923C104317684 @default.
- W1535367923 hasConceptScore W1535367923C112243037 @default.
- W1535367923 hasConceptScore W1535367923C181199279 @default.
- W1535367923 hasConceptScore W1535367923C185592680 @default.
- W1535367923 hasConceptScore W1535367923C2775941552 @default.
- W1535367923 hasConceptScore W1535367923C2779876172 @default.
- W1535367923 hasConceptScore W1535367923C2781259782 @default.
- W1535367923 hasConceptScore W1535367923C55493867 @default.
- W1535367923 hasConceptScore W1535367923C60644358 @default.
- W1535367923 hasConceptScore W1535367923C67705224 @default.
- W1535367923 hasConceptScore W1535367923C86803240 @default.
- W1535367923 hasIssue "16" @default.
- W1535367923 hasLocation W15353679231 @default.
- W1535367923 hasOpenAccess W1535367923 @default.
- W1535367923 hasPrimaryLocation W15353679231 @default.
- W1535367923 hasRelatedWork W1465188717 @default.
- W1535367923 hasRelatedWork W1586839000 @default.
- W1535367923 hasRelatedWork W1596154245 @default.
- W1535367923 hasRelatedWork W2000745848 @default.
- W1535367923 hasRelatedWork W2062150787 @default.
- W1535367923 hasRelatedWork W2110807967 @default.
- W1535367923 hasRelatedWork W2416306772 @default.
- W1535367923 hasRelatedWork W88653793 @default.
- W1535367923 hasRelatedWork W960686591 @default.
- W1535367923 hasRelatedWork W2074668588 @default.
- W1535367923 hasVolume "260" @default.
- W1535367923 isParatext "false" @default.
- W1535367923 isRetracted "false" @default.
- W1535367923 magId "1535367923" @default.
- W1535367923 workType "article" @default.