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• W1536054086 startingPage "2431" @default.
• W1536054086 abstract "We developed procedures for large-scale cultivation of Helicobacter pylori in flasks and fermentors. Flasks incubated closed under a microaerophilic gas phase with a cotton plug covered by a plastic bag, followed by removal of the bag after 8 h, gave excellent growth. Growth in a 10-liter fermentor led to excessive foaming if the medium was sparged with gas; silicone- or polyglycol-based antifoaming agents were severely inhibitory. Use of fermentor surface gassing, first with a microaerophilic 6% oxygen gas mixture, then with air, and then with 95% oxygen, allowed the culture to grow to an A600 of 2.5 in < 24 h. This method was modified for scale-up to a 100-liter fermentor." @default.
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• W1536054086 creator A5020926189 @default.
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• W1536054086 date "1995-06-01" @default.
• W1536054086 modified "2023-10-15" @default.
• W1536054086 title "Rapid large-scale growth of Helicobacter pylori in flasks and fermentors" @default.
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• W1536054086 doi "https://doi.org/10.1128/aem.61.6.2431-2435.1995" @default.
• W1536054086 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/167517" @default.
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