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- W1536695423 abstract "This chapter discusses the expanded-bed adsorption process for protein capture. In the expanded-bed mode, the starting protein solution is pumped upward through a bed of adsorbent beads that are constrained by a flow adaptor. As a result of the upward flow and the properties of the beads, the bed expands as spaces open up between the beads. If the physical properties of the beads are significantly different from those of the particles in the feedstock, the particles can pass freely through the bed without being trapped. An effective process, which means the formation of a stable expanded bed, depends on parameters such as the viscosity, ionic strength, sohds content, and pH of the feedstock as well as the linear flow rate. The expanded-bed operation offers an efficient alternative to the conventional protein capturing process. The latter requires that the starting product solution be centrifuged, clarified, and at times, dialyzed, before it can be adsorbed on a packed column. In contrast, capturing the desired product by the expanded-bed method is simpler because the crude starting solution is pumped directly on the adsorbent." @default.
- W1536695423 created "2016-06-24" @default.
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- W1536695423 date "2000-01-01" @default.
- W1536695423 modified "2023-10-18" @default.
- W1536695423 title "Expanded-bed adsorption process for protein capture" @default.
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- W1536695423 doi "https://doi.org/10.1016/s0149-6395(00)80058-6" @default.
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