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• W1536710835 abstract "The polarographic behaviour of DNA extracted from the calf thymus has been studied using phase sensitive a.c. polarography. The conditions of adsorption on the dropping mercury electrode have been studied in terms of the conditions of medium (ionic strength, pH) and of the molecular weight of DNA. The ionic strength plays a deciding role in the conditions of adsorption. The adsorption also depends on the form of the macromolecule. When it is in rod form (low molecular weight) the adsorption occurs parallel to the charged surface. When it adopts a wormlike form, loops are formed which extend into the solution. The adsorption does not depend on the nature of the monovalent counterion thus the adsorption is identical in Na + and NH 4 + media. The polarograms of native and denatured DNA present an initial rounded peak situated at the limit of the adsorption zone. The native DNA is characterised by a second peak which appears, whatever the medium and the molecular weight of the DNA may be, on the recordings of the in-phase component of the current. This peak is situated at a more positive potential than that which is normally characteristic of the denatured DNA. The characteristics of the peaks of the native DNA have been specified. The first peak corresponds to the desorption of a certain number of adsorbed elements of the macromolecule. The second peak changes its properties according to the pH. In a slightly acid medium, it corresponds to the reduction of the adsorbed bases without desorption of the reduced products. The zones of reduction are the locally destabilized, i.e. the A-T rich regions of the DNA. In alkaline medium it corresponds to the desorption of the macromolecule. A general schema of the behaviour of native DNA on the dropping mercury electrode is proposed." @default.
• W1536710835 created "2016-06-24" @default.
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• W1536710835 date "1976-02-01" @default.
• W1536710835 modified "2023-09-26" @default.
• W1536710835 title "Electrochemical investigation of nucleic acid behaviour" @default.
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• W1536710835 doi "https://doi.org/10.1016/s0022-0728(76)80050-2" @default.
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