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• W1536884364 startingPage "4895" @default.
• W1536884364 abstract "Using pulsed-field gel electrophoresis, we studied the chromosomes of spotted fever group rickettsiae. We digested the DNA of 16 species currently known to belong to this group with SmaI, EagI, and BssHII. The genome size of 13 rickettsiae was between 1,200 and 1,300 kb. Rickettsia massiliae and R. helvetica genome sizes were 1,370 and 1,397 kb, respectively, and that of R. bellii was 1,660 kb. It was possible to obtain distinctive patterns for each species, but in R. conorii, 10 isolates exhibited the same profiles, showing that pulsed-field gel electrophoresis is a good interspecies identification tool. We achieved a phylogenetic analysis of these bacteria by using the Dice coefficient and UPGMA and Package Philip programming. We established a dendrogram of the genetic relationships between the different species showing the existence of a cluster in the spotted fever group rickettsiae including R. conorii, R. rickettsii, R. parkeri, R. sibirica, R. africae, R. slovaca, Thai tick typhus rickettsia, and Israeli tick typhus rickettsia. We located three genes previously cloned and sequenced (genes encoding the R. rickettsii surface proteins of 120 and 190 kDa and the R. prowazekii citrate synthase gene), using Southern hybridization. The genes encoding citrate synthase and the surface protein of 190 kDa were usually located on the same band, and it is hypothesized that they are relatively close on the chromosome." @default.
• W1536884364 created "2016-06-24" @default.
• W1536884364 creator A5050060169 @default.
• W1536884364 creator A5074730004 @default.
• W1536884364 date "1993-08-01" @default.
• W1536884364 modified "2023-09-24" @default.
• W1536884364 title "Genotypic identification and phylogenetic analysis of the spotted fever group rickettsiae by pulsed-field gel electrophoresis" @default.
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• W1536884364 doi "https://doi.org/10.1128/jb.175.15.4895-4904.1993" @default.
• W1536884364 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/204943" @default.
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