Matches in SemOpenAlex for { <https://semopenalex.org/work/W1537213032> ?p ?o ?g. }
- W1537213032 endingPage "1179" @default.
- W1537213032 startingPage "1172" @default.
- W1537213032 abstract "In the rat liver homogenate, maximal protein kinase C activity was found at two calcium concentrations (1.75 and 3.5 mM). Subcellular fractionation of the liver homogenate revealed that the protein kinase C activity requiring 1.75 mM calcium was present only in the cytosolic and particulate subcellular fractions. The protein kinase C activity requiring 3.5 mM calcium concentration was mainly located in the rat liver nuclei preparation. About 19% of the liver homogenate protein kinase C activity requiring 3.5 mM calcium was present in the nuclei. Goat anti-rat brain protein kinase C antibodies revealed a single immunoreactive band at 80-82 kDa in the rat liver nuclear, particulate, or cytosolic fractions. Based on the ratio of plasma membrane marker enzyme activity determined in the nuclear preparation, the purity of the isolated nuclei was ascertained. Rat liver nuclear protein kinase C activity has been partially purified. The purification steps sequentially employed were Triton X-100 extraction of isolated nuclei, DEAE-cellulose chromatography, Phenyl-Superose, and Mono Q (fast protein liquid) chromatography. The final purification step revealed, by silver nitrate staining on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, two protein bands at 80 and 66 kDa, respectively. These findings provide definitive data regarding the nuclear location of protein kinase C. The nuclear location of protein kinase C may lead to an understanding of the molecular pathway involved in signal transduction from the plasma membrane to the nucleus." @default.
- W1537213032 created "2016-06-24" @default.
- W1537213032 creator A5004336882 @default.
- W1537213032 creator A5015396394 @default.
- W1537213032 creator A5035007870 @default.
- W1537213032 creator A5036534144 @default.
- W1537213032 creator A5042715380 @default.
- W1537213032 creator A5051790895 @default.
- W1537213032 creator A5053623432 @default.
- W1537213032 date "1989-01-01" @default.
- W1537213032 modified "2023-09-26" @default.
- W1537213032 title "Protein Kinase C Located in Rat Liver Nuclei" @default.
- W1537213032 cites W1042903243 @default.
- W1537213032 cites W121512958 @default.
- W1537213032 cites W1436936464 @default.
- W1537213032 cites W1480675692 @default.
- W1537213032 cites W1496172532 @default.
- W1537213032 cites W1555146912 @default.
- W1537213032 cites W1561753405 @default.
- W1537213032 cites W1568191878 @default.
- W1537213032 cites W1569108584 @default.
- W1537213032 cites W1588018069 @default.
- W1537213032 cites W1594758412 @default.
- W1537213032 cites W1598863867 @default.
- W1537213032 cites W1964839077 @default.
- W1537213032 cites W1970441944 @default.
- W1537213032 cites W1973005880 @default.
- W1537213032 cites W1978060911 @default.
- W1537213032 cites W1980854090 @default.
- W1537213032 cites W1982152509 @default.
- W1537213032 cites W1987722323 @default.
- W1537213032 cites W2001668713 @default.
- W1537213032 cites W2004917770 @default.
- W1537213032 cites W2005525303 @default.
- W1537213032 cites W2020641276 @default.
- W1537213032 cites W2021144709 @default.
- W1537213032 cites W2022342758 @default.
- W1537213032 cites W2036007824 @default.
- W1537213032 cites W2036979250 @default.
- W1537213032 cites W2037673705 @default.
- W1537213032 cites W2047743040 @default.
- W1537213032 cites W2054800842 @default.
- W1537213032 cites W2057094603 @default.
- W1537213032 cites W2061628918 @default.
- W1537213032 cites W2062499314 @default.
- W1537213032 cites W2068877244 @default.
- W1537213032 cites W2072436875 @default.
- W1537213032 cites W2085674119 @default.
- W1537213032 cites W2087236026 @default.
- W1537213032 cites W2092251053 @default.
- W1537213032 cites W2095296343 @default.
- W1537213032 cites W2100837269 @default.
- W1537213032 cites W2101108802 @default.
- W1537213032 cites W2112365969 @default.
- W1537213032 cites W2154124833 @default.
- W1537213032 cites W2160733363 @default.
- W1537213032 cites W2401266140 @default.
- W1537213032 cites W4293247451 @default.
- W1537213032 doi "https://doi.org/10.1016/s0021-9258(19)85068-8" @default.
- W1537213032 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2910849" @default.
- W1537213032 hasPublicationYear "1989" @default.
- W1537213032 type Work @default.
- W1537213032 sameAs 1537213032 @default.
- W1537213032 citedByCount "137" @default.
- W1537213032 countsByYear W15372130322013 @default.
- W1537213032 countsByYear W15372130322014 @default.
- W1537213032 countsByYear W15372130322015 @default.
- W1537213032 crossrefType "journal-article" @default.
- W1537213032 hasAuthorship W1537213032A5004336882 @default.
- W1537213032 hasAuthorship W1537213032A5015396394 @default.
- W1537213032 hasAuthorship W1537213032A5035007870 @default.
- W1537213032 hasAuthorship W1537213032A5036534144 @default.
- W1537213032 hasAuthorship W1537213032A5042715380 @default.
- W1537213032 hasAuthorship W1537213032A5051790895 @default.
- W1537213032 hasAuthorship W1537213032A5053623432 @default.
- W1537213032 hasBestOaLocation W15372130321 @default.
- W1537213032 hasConcept C153911025 @default.
- W1537213032 hasConcept C185592680 @default.
- W1537213032 hasConcept C55493867 @default.
- W1537213032 hasConcept C86803240 @default.
- W1537213032 hasConcept C95444343 @default.
- W1537213032 hasConceptScore W1537213032C153911025 @default.
- W1537213032 hasConceptScore W1537213032C185592680 @default.
- W1537213032 hasConceptScore W1537213032C55493867 @default.
- W1537213032 hasConceptScore W1537213032C86803240 @default.
- W1537213032 hasConceptScore W1537213032C95444343 @default.
- W1537213032 hasIssue "2" @default.
- W1537213032 hasLocation W15372130321 @default.
- W1537213032 hasOpenAccess W1537213032 @default.
- W1537213032 hasPrimaryLocation W15372130321 @default.
- W1537213032 hasRelatedWork W1531601525 @default.
- W1537213032 hasRelatedWork W1990781990 @default.
- W1537213032 hasRelatedWork W2344122601 @default.
- W1537213032 hasRelatedWork W2606230654 @default.
- W1537213032 hasRelatedWork W2607424097 @default.
- W1537213032 hasRelatedWork W2748952813 @default.
- W1537213032 hasRelatedWork W2899084033 @default.
- W1537213032 hasRelatedWork W2948807893 @default.