FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1537652082> ?p ?o ?g. }

• W1537652082 endingPage "2095" @default.
• W1537652082 startingPage "2087" @default.
• W1537652082 abstract "Affinity-purified polyclonal antibodies, raised against two synthetic peptides corresponding to the R domain and the C terminus of the human cystic fibrosis transmembrane conductance regulator (CFTR), were used to characterize and localize the protein in human epithelial cells. Employing an immunoblotting technique that ensures efficient detection of large hydrophobic proteins, both antibodies recognized and approximately 180-kDa protein in cell lysates and isolated membranes of airway epithelial cells from normal and cystic fibrosis (CF) patients and of T84 colon carcinoma cells. Reactivity with the anti-C terminus antibody, but not with the anti-R domain antibody, was eliminated by limited carboxypeptidase Y digestion. When normal CFTR cDNA was overexpressed via a retroviral vector in CF or normal airway epithelial cells or in mouse fibroblasts, the protein produced had an apparent molecular mass of about 180 kDa. The CFTR expressed in insect (Sf9) cells by a baculovirus vector had a molecular mass of about 140 kDa, probably representing a nonglycosylated form. The CFTR in epithelial cells appears to exist in several forms. N-glycosidase treatment of T84 cell membranes reduces the apparent molecular mass of the major CFTR band from 180 kDa to 140 kDa, but a fraction of the T84 cell CFTR could not be deglycosylated, and the CFTR in airway epithelial cell membranes could not be deglycosylated either. Moreover, wheat germ agglutinin absorbs the majority of the CFTR from detergent-solubilized T84 cell membranes but not from airway cell membranes. The CFTR in all epithelial cell types was found to be an integral membrane protein not solubilized by high salt or lithium diiodosalicylate treatment. Sucrose density gradient fractionation of crude membranes prepared from the airway epithelial cells, previously surface-labeled by enzymatic galactosidation, showed a plasma membrane localization for both the normal CFTR and the CFTR carrying the Phe508 deletion (delta F 508). The CFTR in all cases co-localized with the Na+, K(+)-ATPase and the plasma membrane calcium ATPase, while the endoplasmic reticulum calcium ATPase and mitochondrial membrane markers were enriched at higher sucrose densities. Thus, the CFTR appears to be localized in the plasma membrane both in normal and delta F 508 CF epithelial cells." @default.
• W1537652082 created "2016-06-24" @default.
• W1537652082 creator A5001166056 @default.
• W1537652082 creator A5002200455 @default.
• W1537652082 creator A5002750883 @default.
• W1537652082 creator A5015926167 @default.
• W1537652082 creator A5023847753 @default.
• W1537652082 creator A5026053999 @default.
• W1537652082 creator A5030180282 @default.
• W1537652082 creator A5034821657 @default.
• W1537652082 creator A5049159831 @default.
• W1537652082 creator A5077020962 @default.
• W1537652082 date "1992-01-01" @default.
• W1537652082 modified "2023-09-27" @default.
• W1537652082 title "Biochemical characterization of the cystic fibrosis transmembrane conductance regulator in normal and cystic fibrosis epithelial cells." @default.
• W1537652082 cites W1198166609 @default.
• W1537652082 cites W1497503028 @default.
• W1537652082 cites W1553673732 @default.
• W1537652082 cites W1560326407 @default.
• W1537652082 cites W175263069 @default.
• W1537652082 cites W1786021994 @default.
• W1537652082 cites W1969697265 @default.
• W1537652082 cites W1973868711 @default.
• W1537652082 cites W1975971473 @default.
• W1537652082 cites W1979496441 @default.
• W1537652082 cites W1982071077 @default.
• W1537652082 cites W1984367265 @default.
• W1537652082 cites W1990637933 @default.
• W1537652082 cites W1995240556 @default.
• W1537652082 cites W2003724450 @default.
• W1537652082 cites W2010416376 @default.
• W1537652082 cites W2016013368 @default.
• W1537652082 cites W2016532191 @default.
• W1537652082 cites W2027282091 @default.
• W1537652082 cites W2036774130 @default.
• W1537652082 cites W2039599894 @default.
• W1537652082 cites W2049827739 @default.
• W1537652082 cites W2053418558 @default.
• W1537652082 cites W2057474701 @default.
• W1537652082 cites W2090784961 @default.
• W1537652082 cites W2100432609 @default.
• W1537652082 cites W2125391335 @default.
• W1537652082 cites W2269547969 @default.
• W1537652082 doi "https://doi.org/10.1016/s0021-9258(18)46056-5" @default.
• W1537652082 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1370488" @default.
• W1537652082 hasPublicationYear "1992" @default.
• W1537652082 type Work @default.
• W1537652082 sameAs 1537652082 @default.
• W1537652082 citedByCount "89" @default.
• W1537652082 countsByYear W15376520822015 @default.
• W1537652082 countsByYear W15376520822016 @default.
• W1537652082 countsByYear W15376520822017 @default.
• W1537652082 countsByYear W15376520822020 @default.
• W1537652082 countsByYear W15376520822021 @default.
• W1537652082 countsByYear W15376520822023 @default.
• W1537652082 crossrefType "journal-article" @default.
• W1537652082 hasAuthorship W1537652082A5001166056 @default.
• W1537652082 hasAuthorship W1537652082A5002200455 @default.
• W1537652082 hasAuthorship W1537652082A5002750883 @default.
• W1537652082 hasAuthorship W1537652082A5015926167 @default.
• W1537652082 hasAuthorship W1537652082A5023847753 @default.
• W1537652082 hasAuthorship W1537652082A5026053999 @default.
• W1537652082 hasAuthorship W1537652082A5030180282 @default.
• W1537652082 hasAuthorship W1537652082A5034821657 @default.
• W1537652082 hasAuthorship W1537652082A5049159831 @default.
• W1537652082 hasAuthorship W1537652082A5077020962 @default.
• W1537652082 hasBestOaLocation W15376520821 @default.
• W1537652082 hasConcept C104317684 @default.
• W1537652082 hasConcept C12554922 @default.
• W1537652082 hasConcept C126322002 @default.
• W1537652082 hasConcept C142724271 @default.
• W1537652082 hasConcept C170493617 @default.
• W1537652082 hasConcept C171250308 @default.
• W1537652082 hasConcept C185592680 @default.
• W1537652082 hasConcept C192562407 @default.
• W1537652082 hasConcept C24530287 @default.
• W1537652082 hasConcept C2776938444 @default.
• W1537652082 hasConcept C2778428886 @default.
• W1537652082 hasConcept C2780559512 @default.
• W1537652082 hasConcept C2780841128 @default.
• W1537652082 hasConcept C529295009 @default.
• W1537652082 hasConcept C55493867 @default.
• W1537652082 hasConcept C6929976 @default.
• W1537652082 hasConcept C71924100 @default.
• W1537652082 hasConcept C86803240 @default.
• W1537652082 hasConcept C95444343 @default.
• W1537652082 hasConceptScore W1537652082C104317684 @default.
• W1537652082 hasConceptScore W1537652082C12554922 @default.
• W1537652082 hasConceptScore W1537652082C126322002 @default.
• W1537652082 hasConceptScore W1537652082C142724271 @default.
• W1537652082 hasConceptScore W1537652082C170493617 @default.
• W1537652082 hasConceptScore W1537652082C171250308 @default.
• W1537652082 hasConceptScore W1537652082C185592680 @default.
• W1537652082 hasConceptScore W1537652082C192562407 @default.
• W1537652082 hasConceptScore W1537652082C24530287 @default.
• W1537652082 hasConceptScore W1537652082C2776938444 @default.
• W1537652082 hasConceptScore W1537652082C2778428886 @default.
• W1537652082 hasConceptScore W1537652082C2780559512 @default.

• next