FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1538266938> ?p ?o ?g. }

• W1538266938 endingPage "3025" @default.
• W1538266938 startingPage "3015" @default.
• W1538266938 abstract "Abstract The enzyme that catalyzes the removal of a two-carbon compound from 2-amino-4-hydroxy-6-(d-erythro-1',2',3'-trihydroxypropyl)-7,8-dihydropteridine (known by the trivial name of dihydroneopterin) to yield 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine, has been purified by approximately 1100-fold from extracts of Escherichia coli B. The two-carbon compound formed as a product has been identified as glycolaldehyde. Since the reaction is similar to those reactions catalyzed by aldolases, the enzyme has been named dihydroneopterin aldolase. The enzyme is relatively heat-stable in that it can withstand a temperature of 100° for 5 min with virtually no loss of activity. The enzyme functions optimally at pH 9.6. The addition of chelating agents such as EDTA markedly increases the activity of the enzyme. The Km for dihydroneopterin is approximately 9 µm. The enzyme cannot use the following compounds as substrates in place of dihydroneopterin: neopterin, tetrahydroneopterin, dihydroneopterin monophosphate, and dihydroneopterin triphosphate. The product of the reaction, 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine, is a potent inhibitor of the reaction. Other dihydropteridines, including dihydropteroic acid and dihydrofolic acid, are less potent inhibitors. It was found that purified dihydroneopterin aldolase could use as substrate an epimer of dihydroneopterin, i.e. 2-amino-4-hydroxy-(6-l-threo-trihydroxypropyl)-7,8-dihydropteridine, but the corresponding d-threo and l-erythro compounds could not be utilized, although the l-erythro compound could be utilized for the synthesis of 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine in the presence of purified dihydroneopterin aldolase and another enzyme fraction obtained from extracts of E. coli. This suggests that E. coli contains an enzyme that catalyzes the epimerization of the l-erythro compound to a compound that can be used directly as substrate by the purified aldolase (presumably the corresponding l-threo compound)." @default.
• W1538266938 created "2016-06-24" @default.
• W1538266938 creator A5038984703 @default.
• W1538266938 creator A5053845595 @default.
• W1538266938 date "1970-06-01" @default.
• W1538266938 modified "2023-10-12" @default.
• W1538266938 title "The Biosynthesis of Folic Acid" @default.
• W1538266938 cites W1503770775 @default.
• W1538266938 cites W1517002953 @default.
• W1538266938 cites W157359994 @default.
• W1538266938 cites W1578847828 @default.
• W1538266938 cites W1588233911 @default.
• W1538266938 cites W163127642 @default.
• W1538266938 cites W1652310094 @default.
• W1538266938 cites W1775749144 @default.
• W1538266938 cites W1974837951 @default.
• W1538266938 cites W2024618059 @default.
• W1538266938 cites W2026912546 @default.
• W1538266938 cites W2027069937 @default.
• W1538266938 cites W2028845844 @default.
• W1538266938 cites W2065627978 @default.
• W1538266938 cites W2080486100 @default.
• W1538266938 cites W2083587155 @default.
• W1538266938 cites W2087213921 @default.
• W1538266938 cites W2111819355 @default.
• W1538266938 cites W2126614046 @default.
• W1538266938 cites W2134812258 @default.
• W1538266938 cites W2316472358 @default.
• W1538266938 cites W2334435328 @default.
• W1538266938 cites W24073088 @default.
• W1538266938 cites W4255074928 @default.
• W1538266938 cites W63225481 @default.
• W1538266938 cites W990606643 @default.
• W1538266938 doi "https://doi.org/10.1016/s0021-9258(18)63090-x" @default.
• W1538266938 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4912541" @default.
• W1538266938 hasPublicationYear "1970" @default.
• W1538266938 type Work @default.
• W1538266938 sameAs 1538266938 @default.
• W1538266938 citedByCount "53" @default.
• W1538266938 countsByYear W15382669382012 @default.
• W1538266938 countsByYear W15382669382014 @default.
• W1538266938 countsByYear W15382669382015 @default.
• W1538266938 countsByYear W15382669382017 @default.
• W1538266938 countsByYear W15382669382018 @default.
• W1538266938 countsByYear W15382669382020 @default.
• W1538266938 countsByYear W15382669382022 @default.
• W1538266938 countsByYear W15382669382023 @default.
• W1538266938 crossrefType "journal-article" @default.
• W1538266938 hasAuthorship W1538266938A5038984703 @default.
• W1538266938 hasAuthorship W1538266938A5053845595 @default.
• W1538266938 hasBestOaLocation W15382669381 @default.
• W1538266938 hasConcept C104317684 @default.
• W1538266938 hasConcept C126322002 @default.
• W1538266938 hasConcept C185592680 @default.
• W1538266938 hasConcept C2992130864 @default.
• W1538266938 hasConcept C553450214 @default.
• W1538266938 hasConcept C55493867 @default.
• W1538266938 hasConcept C70721500 @default.
• W1538266938 hasConcept C71924100 @default.
• W1538266938 hasConcept C86803240 @default.
• W1538266938 hasConceptScore W1538266938C104317684 @default.
• W1538266938 hasConceptScore W1538266938C126322002 @default.
• W1538266938 hasConceptScore W1538266938C185592680 @default.
• W1538266938 hasConceptScore W1538266938C2992130864 @default.
• W1538266938 hasConceptScore W1538266938C553450214 @default.
• W1538266938 hasConceptScore W1538266938C55493867 @default.
• W1538266938 hasConceptScore W1538266938C70721500 @default.
• W1538266938 hasConceptScore W1538266938C71924100 @default.
• W1538266938 hasConceptScore W1538266938C86803240 @default.
• W1538266938 hasIssue "11" @default.
• W1538266938 hasLocation W15382669381 @default.
• W1538266938 hasOpenAccess W1538266938 @default.
• W1538266938 hasPrimaryLocation W15382669381 @default.
• W1538266938 hasRelatedWork W1531601525 @default.
• W1538266938 hasRelatedWork W2319480705 @default.
• W1538266938 hasRelatedWork W2384464875 @default.
• W1538266938 hasRelatedWork W2606230654 @default.
• W1538266938 hasRelatedWork W2607424097 @default.
• W1538266938 hasRelatedWork W2748952813 @default.
• W1538266938 hasRelatedWork W2899084033 @default.
• W1538266938 hasRelatedWork W2948807893 @default.
• W1538266938 hasRelatedWork W4387497383 @default.
• W1538266938 hasRelatedWork W2778153218 @default.
• W1538266938 hasVolume "245" @default.
• W1538266938 isParatext "false" @default.
• W1538266938 isRetracted "false" @default.
• W1538266938 magId "1538266938" @default.
• W1538266938 workType "article" @default.