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- W1538644515 abstract "Publisher Summary Genetic fingerprinting techniques are excellently suited to comparison of large numbers of samples. Genetic fingerprinting of microbial communities provides banding patterns or profiles that reflect the genetic diversity of the community. Denaturing gradient gel electrophoresis (DGGE) of PCRamplified gene fragments is one of the genetic fingerprinting techniques used in microbial ecology. In DGGE, similar-sized DNA fragments are separated in a gradient of DNA denaturants according to differences in sequence. A variant of DGGE, temperature gradient gel electrophoresis (TGGE), makes use of a temperature gradient to separate gene fragments. DGGE is relatively easy to perform and is especially suited to the analysis of multiple samples. Since its introduction into microbial ecology, it has been adapted in many laboratories as a convenient tool for the assessment of microbial diversity in natural samples. It has been shown by several studies that the approach is reproducible and sensitive. Other new fingerprinting techniques, such as automated T-RFLP might be more sensitive but identification of predominant community members still requires cloning and sequencing of PCR products. A potential future development in PCR-DGGE fingerprinting might be to use fluorescently labelled PCR primers, which might (1) make staining of gels unnecessary, and (2) make it possible to add intra-lane standards with a different fluorochrome, facilitating gel-to-gel comparisons." @default.
- W1538644515 created "2016-06-24" @default.
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- W1538644515 creator A5045799758 @default.
- W1538644515 date "2001-01-01" @default.
- W1538644515 modified "2023-10-12" @default.
- W1538644515 title "Denaturing gradient gel electrophoresis in marine microbial ecology" @default.
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- W1538644515 doi "https://doi.org/10.1016/s0580-9517(01)30057-0" @default.
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