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- W1538800028 abstract "In bacteria, transcription and DNA replication occur concurrently, inevitably causing replication-transcription conflicts. Conflicts can occur both co-directionally (leading strand genes) and head-on (lagging strand genes), leading to replisome stalling and restart. Because the majority of genes are co-oriented with replication in most bacteria, co-directional conflicts are expected to be highly prevalent, impacting replication speed and genomic stability. Consequently, mechanisms that deal with these conflicts should be critical for the timely and accurate duplication of genomes. Here we report that the essential Bacillus subtilis accessory helicase, PcrA, mitigates both co-directional and head-on conflicts between the replication and transcription machineries. Using ChIP-seq, we have identified genomic regions where PcrA associates and facilitates replication progression. We find that PcrA predominantly associates with co-directional rDNA and tRNA genes, in a transcription-dependent manner. When PcrA is depleted, and only when transcription is active, replisome stalling and restart increases in the same areas. PcrA also reduces transcription-induced replication stalling and restart at other regions, including some head-on genes. Differential levels of PcrA at engineered head-on and co-directional conflicts indicate that its association and activity correlate with conflict severity, rather than transcription levels. These results show that PcrA reduces replisomes stalling, and restart, caused by head-on and co-directional replication-transcription conflicts. Furthermore, these data indicate that during vegetative growth, and in the absence of exogenous stress, the most difficult regions to replicate are co-directionally transcribed genes. Both the genome co-orientation bias and these accessory helicases are widely conserved, suggesting that our results are relevant for many other bacterial species. Grant Funding Source: NIH" @default.
- W1538800028 created "2016-06-24" @default.
- W1538800028 creator A5035459459 @default.
- W1538800028 creator A5086288375 @default.
- W1538800028 date "2014-04-01" @default.
- W1538800028 modified "2023-09-27" @default.
- W1538800028 title "The B. subtilis accessory helicase PcrA facilitates replication through transcription units genome‐wide (LB126)" @default.
- W1538800028 doi "https://doi.org/10.1096/fasebj.28.1_supplement.lb126" @default.
- W1538800028 hasPublicationYear "2014" @default.
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