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• W1539016845 abstract "Objectives: To study the altered expression of TWIK-related arachidonic acid-activated K+ channel in the L6–S1 spinal cord of rats after complete bladder outlet obstruction, and to investigate the role of TWIK-related arachidonic acid-activated K+ channel in the neurogenic mechanism of bladder dysfunction. Methods: Female Sprague–Dawley rats were randomly divided into a complete bladder outlet obstruction group and a sham-operated control group. Cystometry was carried out and tissues of L6–S1 spinal cord were obtained for detection of TWIK-related arachidonic acid-activated K+ channel mRNA and protein by real-time polymerase chain reaction, western blot and immunohistochemistry. Results: The bladder outlet obstruction rat model was established. Real-time polymerase chain reaction, western blot and immunohistochemistry showed that the expression of TWIK-related arachidonic acid-activated K+ channel was lower in the L6–S1 spinal cord of the bladder outlet obstruction rats, compared with the control rats. Conclusions: Downregulation of TWIK-related arachidonic acid-activated K+ channel might enhance the excitability of the neurons and increase the sensitivity of the bladder, probably providing a new study model of overactive bladder secondary to bladder outlet obstruction." @default.
• W1539016845 created "2016-06-24" @default.
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• W1539016845 date "2012-06-19" @default.
• W1539016845 modified "2023-09-27" @default.
• W1539016845 title "Downregulation of TWIK-related arachidonic acid-activated K+channel in the spinal cord of rats after complete bladder outlet obstruction" @default.
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• W1539016845 doi "https://doi.org/10.1111/j.1442-2042.2012.03072.x" @default.
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