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• W1539046830 abstract "Snake venom is a potentially lethal and complex mixture of functionally diverse proteins. The complex nature of the venom toxin exerts limitations towards the development of toxin targeted therapy. Conventional antivenom/antisera of equine origin also pose the risks of anaphylaxis and serum sickness in the envenomated snake bite victims. Previous investigations have revealed that the active subunits of the toxic venom are potentiated by the non-toxic subunits which act as chaperones, inorder to increase the specificity and stability of the lethal components (Gutierrez et al., 2005; Swenson and Markland, 2005; Kamiguti, 2005; White, 2005). In this context, metallic elements present in the venom function as co factors in the enzymes activity as well as in enabling the cellular infiltration of the venom toxin to cause vascular and circulatory system disorders. In the present study, an attempt was made to determine the effect of a chelator compound like EDTA in the cytotoxic action of Cobra venom (CV) and Russell’s viper venom (RV) on Sp2/0 cell lines. Since these venom toxins exhibit calcium dependent cytotoxicity, (Moral et al 2006) the effect of EDTA on the above property was also determined. EDTA is a polyamino carboxylic acid and its water soluble nature enables it as a best chelating agent capable of combining stoichiometrically almost all divalent and trivalent metal ions. Since calcium plays a key role in the haemorrhage and proteolysis, the neutralizing action of EDTA would be of importance in the attenuation of snake venom toxicity. Cytotoxicity assay by Tryphan blue exclusion method was carried out using various concentrations of Russell’s viper venom and cobra venom (Obtained from Irula’s Society, Kancheepuram, Tamil Nadu, India) on Sp2/O cell lines (Aldred and Cooke 1983 ; Lipps 1999).0.1 million of myeloma cells were added to each well of 96 well plate, along the row in duplicate. To these cells, venom was serially diluted from the concentration of 1500ng to 300ng for CV and 20µg to 2.5µg RV. The cells were incubated for 2 hrs at 37 o C. After incubation, the viability of the cells were measured using Trypan Blue. Similarly, the EDTA was standardized and a concentration of 10mM was mixed along with the venom sample for cytotoxicity. To check the chelating property of EDTA, CaCl2 was also added to the cells at the equimolar concentration of the EDTA. The results of cytotoxicity effect of venoms, their property in the presence of EDTA, CaCl2 and/ EDTA + CaCl2 were illustrated." @default.
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• W1539046830 date "2010-12-25" @default.
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• W1539046830 title "A cross reactive study on attenuation of Cobra and Russell’s viper venoms cytotoxicity by EDTA using Sp2/0 myeloma cells" @default.
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