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- W1539090868 abstract "Key events in cellular trafficking occur at the cell surface, and it is desirable to visualize these events without interference from other regions deeper within. This review describes a microscopy technique based on total internal reflection fluorescence which is well suited for optical sectioning at cell-substrate regions with an unusually thin region of fluorescence excitation. The technique has many other applications as well, most notably for studying biochemical kinetics and single biomolecule dynamics at surfaces. A brief summary of these applications is provided, followed by presentations of the physical basis for the technique and the various ways to implement total internal reflection fluorescence in a standard fluorescence microscope." @default.
- W1539090868 created "2016-06-24" @default.
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- W1539090868 date "2001-11-01" @default.
- W1539090868 modified "2023-10-18" @default.
- W1539090868 title "Total Internal Reflection Fluorescence Microscopy in Cell Biology" @default.
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- W1539090868 doi "https://doi.org/10.1034/j.1600-0854.2001.21104.x" @default.
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