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• W1539103535 abstract "A maize prolamin box (P-box)-binding factor (PBF-1) has been purified and characterized from immature endosperm tissue. PBF-1 has a molecular weight of 38 kDa. It is detected only in endosperm, but not in root or leaf tissues, consistent with its tissue-specific function. Site-directed mutagenesis experiments reveal that both the P-box and its flanking sequences are important for PBF-1 DNA binding. Developmental studies show that PBF-1 accumulates in the endosperm from 8 to at least 30 days after pollination (DAP). From 16 to 24 DAP, however, multiple shifted bands of protein(s)–DNA complexes can be observed, which correlate with an increase in zein gene expression. PBF-1 can also bind to the P-box from `22-kDa' and `19-kDa' zein promoters, but at a lower affinity than to the `27-kDa' zein promoter. The effects of protein dephosphorylation and zinc ion chelators on PBF-1 DNA binding activity are also shown. A model is proposed where PBF-1 serves as a `recruiter' of class-specific transcription factors like Opaque2 (O2)." @default.
• W1539103535 created "2016-06-24" @default.
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• W1539103535 date "1998-11-01" @default.
• W1539103535 modified "2023-09-25" @default.
• W1539103535 title "Characterization of the maize prolamin box-binding factor-1 (PBF-1) and its role in the developmental regulation of the zein multigene family1Published in conjunction with A Wisconsin Gathering Honoring Waclaw Szybalski on occasion of his 75th year and 20 years of Editorship-in-Chief of Gene, 10–11 August 1997, University of Wisconsin, Madison, WI, USA.1" @default.
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• W1539103535 doi "https://doi.org/10.1016/s0378-1119(98)00244-3" @default.
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