FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1539176720> ?p ?o ?g. }

• W1539176720 endingPage "16732" @default.
• W1539176720 startingPage "16726" @default.
• W1539176720 abstract "Pyrococcus furiosus is a strictly anaerobic archaeon that grows optimally at 100 degrees C by a fermentative-type metabolism in which complex peptide mixtures such as yeast extract and Tryptone, and also certain sugars, are oxidized to organic acids, H2 and CO2. Enzymes involved in the utilization of peptides such as proteases, aromatic amino transferases, and glutamate dehydrogenase have been previously purified from this organism. It is shown here that P. furiosus also contains significant cytoplasmic concentrations of a new enzyme termed indolepyruvate ferredoxin oxidoreductase (IOR). This catalyzes the oxidative decarboxylation of aryl pyruvates, which are generated by the transamination of aromatic amino acids, to the corresponding aryl acetyl-CoA. IOR is a tetramer (alpha 2 beta 2) of two identical subunits (66,000 and 23,000 Da) with a molecular weight of 180,000. The enzyme contains one molecule of thiamine pyrophosphate and four [4Fe-4S]2+,1+ and one [3Fe-4S]0,1+ cluster, as determined by iron analyses and EPR spectroscopy. Significant amounts of other metals such as copper and zinc were not detected. IOR was virtually inactive at 25 degrees C and exhibited optimal activity above 90 degrees C (at pH 8.0) and at pH 8.5-10.5 (at 80 degrees C). The enzyme was sensitive to inactivation by O2, losing 50% of its activity after exposure to air for 20 min at 23 degrees C, and was quite thermostable, with a half-life of activity at 80 degrees C (under anaerobic conditions) of about 80 min. The Km values (in microM) for indolepyruvate, p-hydroxyphenylpyruvate, phenylpyruvate, CoASH, and P. furiosus ferredoxin, the physiological electron carrier, were 250, 110, 90, 17, and 48, respectively. IOR was inhibited by KCN (apparent Ki = 7.5 mM), but not by CO (1 atm). An enzyme analogous to IOR has not been reported previously. Curiously, it has few properties in common with the pyruvate ferredoxin oxidoreductase of P. furiosus, even though the two enzymes catalyze virtually identical reactions. In fact, of known ketoacid oxidoreductases, the catalytic mechanism of IOR appears to be most similar to that of the pyruvate ferredoxin oxidoreductase from the hyperthermophilic bacterium Thermotoga maritima." @default.
• W1539176720 created "2016-06-24" @default.
• W1539176720 creator A5000865211 @default.
• W1539176720 creator A5010547713 @default.
• W1539176720 date "1994-06-01" @default.
• W1539176720 modified "2023-09-30" @default.
• W1539176720 title "Indolepyruvate ferredoxin oxidoreductase from the hyperthermophilic archaeon Pyrococcus furiosus. A new enzyme involved in peptide fermentation" @default.
• W1539176720 cites W1226948650 @default.
• W1539176720 cites W1501025810 @default.
• W1539176720 cites W1515327660 @default.
• W1539176720 cites W1518551135 @default.
• W1539176720 cites W1547929361 @default.
• W1539176720 cites W1558027963 @default.
• W1539176720 cites W1562095280 @default.
• W1539176720 cites W1564565358 @default.
• W1539176720 cites W1615690166 @default.
• W1539176720 cites W1699747285 @default.
• W1539176720 cites W1775749144 @default.
• W1539176720 cites W1783650594 @default.
• W1539176720 cites W1964565731 @default.
• W1539176720 cites W1968652449 @default.
• W1539176720 cites W1969383181 @default.
• W1539176720 cites W2001513148 @default.
• W1539176720 cites W2005553811 @default.
• W1539176720 cites W2009758786 @default.
• W1539176720 cites W2015835796 @default.
• W1539176720 cites W2016546278 @default.
• W1539176720 cites W2019252943 @default.
• W1539176720 cites W2019465070 @default.
• W1539176720 cites W2035626612 @default.
• W1539176720 cites W2046218914 @default.
• W1539176720 cites W2057704466 @default.
• W1539176720 cites W2064763310 @default.
• W1539176720 cites W2067621594 @default.
• W1539176720 cites W2071364006 @default.
• W1539176720 cites W2075040966 @default.
• W1539176720 cites W2075918185 @default.
• W1539176720 cites W2085737824 @default.
• W1539176720 cites W2087875520 @default.
• W1539176720 cites W2095297536 @default.
• W1539176720 cites W2104059719 @default.
• W1539176720 cites W2119586900 @default.
• W1539176720 cites W2130415541 @default.
• W1539176720 cites W2152451259 @default.
• W1539176720 cites W2166865790 @default.
• W1539176720 cites W2179100843 @default.
• W1539176720 cites W2316955142 @default.
• W1539176720 cites W2371465739 @default.
• W1539176720 cites W2409105530 @default.
• W1539176720 cites W4243088827 @default.
• W1539176720 cites W952135609 @default.
• W1539176720 doi "https://doi.org/10.1016/s0021-9258(19)89451-6" @default.
• W1539176720 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8206994" @default.
• W1539176720 hasPublicationYear "1994" @default.
• W1539176720 type Work @default.
• W1539176720 sameAs 1539176720 @default.
• W1539176720 citedByCount "117" @default.
• W1539176720 countsByYear W15391767202012 @default.
• W1539176720 countsByYear W15391767202013 @default.
• W1539176720 countsByYear W15391767202014 @default.
• W1539176720 countsByYear W15391767202015 @default.
• W1539176720 countsByYear W15391767202016 @default.
• W1539176720 countsByYear W15391767202017 @default.
• W1539176720 countsByYear W15391767202018 @default.
• W1539176720 countsByYear W15391767202019 @default.
• W1539176720 countsByYear W15391767202020 @default.
• W1539176720 countsByYear W15391767202021 @default.
• W1539176720 countsByYear W15391767202022 @default.
• W1539176720 countsByYear W15391767202023 @default.
• W1539176720 crossrefType "journal-article" @default.
• W1539176720 hasAuthorship W1539176720A5000865211 @default.
• W1539176720 hasAuthorship W1539176720A5010547713 @default.
• W1539176720 hasBestOaLocation W15391767201 @default.
• W1539176720 hasConcept C100544194 @default.
• W1539176720 hasConcept C104317684 @default.
• W1539176720 hasConcept C134621786 @default.
• W1539176720 hasConcept C181199279 @default.
• W1539176720 hasConcept C185592680 @default.
• W1539176720 hasConcept C2776943354 @default.
• W1539176720 hasConcept C2779268744 @default.
• W1539176720 hasConcept C2779281246 @default.
• W1539176720 hasConcept C550995028 @default.
• W1539176720 hasConcept C55428944 @default.
• W1539176720 hasConcept C55493867 @default.
• W1539176720 hasConcept C79115784 @default.
• W1539176720 hasConceptScore W1539176720C100544194 @default.
• W1539176720 hasConceptScore W1539176720C104317684 @default.
• W1539176720 hasConceptScore W1539176720C134621786 @default.
• W1539176720 hasConceptScore W1539176720C181199279 @default.
• W1539176720 hasConceptScore W1539176720C185592680 @default.
• W1539176720 hasConceptScore W1539176720C2776943354 @default.
• W1539176720 hasConceptScore W1539176720C2779268744 @default.
• W1539176720 hasConceptScore W1539176720C2779281246 @default.
• W1539176720 hasConceptScore W1539176720C550995028 @default.
• W1539176720 hasConceptScore W1539176720C55428944 @default.
• W1539176720 hasConceptScore W1539176720C55493867 @default.
• W1539176720 hasConceptScore W1539176720C79115784 @default.
• W1539176720 hasIssue "24" @default.

• next