Matches in SemOpenAlex for { <https://semopenalex.org/work/W1539180171> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W1539180171 abstract "Abstract The d-alanine carboxypeptidase, the major penicillinbinding component in membranes of Bacillus stearothermophilus, was purified by covalent affinity chromatography. The enzyme was solubilized in the nonionic detergent Triton X-100, bound to a column of a 6-aminopenicillanic acid-Sepharose derivative, and eluted upon treatment of the column with neutral hydroxylamine. The enzyme was thermophilic, possessing a temperature optimum of 55°. Its molecular weight, 46,500, resembled that of the d-alanine carboxypeptidase from Bacillus subtilis. In addition to its carboxypeptidase activity, the enzyme was also capable of carrying out a simple transpeptidation reaction. This reaction, which was penicillin sensitive, involved UDP-N-acetylmuramyl -l - alanyl - d - glutamyl - mesodiaminopimelyl - d - alanyl-d-alanine as transpeptidation donor and either glycine or d-alanine as transpeptidation acceptor. The enzyme, however, did not appear to be the lethal target for β-lactam antibiotics in this organism since the enzyme was 27,000-fold more resistant to cephalothin than was the organism." @default.
- W1539180171 created "2016-06-24" @default.
- W1539180171 creator A5010714518 @default.
- W1539180171 creator A5013759935 @default.
- W1539180171 creator A5040129865 @default.
- W1539180171 date "1974-08-01" @default.
- W1539180171 modified "2023-10-01" @default.
- W1539180171 title "Purification and Characterization of the Thermophilic d-Alanine Carboxypeptidase from Membranes of Bacillus stearothermophilus" @default.
- W1539180171 cites W1484040049 @default.
- W1539180171 cites W1501839223 @default.
- W1539180171 cites W1527635388 @default.
- W1539180171 cites W1530645968 @default.
- W1539180171 cites W1533750326 @default.
- W1539180171 cites W1549019539 @default.
- W1539180171 cites W1550256141 @default.
- W1539180171 cites W1578073842 @default.
- W1539180171 cites W1608216341 @default.
- W1539180171 cites W1625660792 @default.
- W1539180171 cites W1775749144 @default.
- W1539180171 cites W1967736866 @default.
- W1539180171 cites W1984224216 @default.
- W1539180171 cites W1985242603 @default.
- W1539180171 cites W1994578946 @default.
- W1539180171 cites W2002192628 @default.
- W1539180171 cites W2015351127 @default.
- W1539180171 cites W2030330409 @default.
- W1539180171 cites W2032290864 @default.
- W1539180171 cites W2042640333 @default.
- W1539180171 cites W2051832897 @default.
- W1539180171 cites W2058065258 @default.
- W1539180171 cites W2078650511 @default.
- W1539180171 cites W4246090862 @default.
- W1539180171 cites W4254807231 @default.
- W1539180171 doi "https://doi.org/10.1016/s0021-9258(19)42401-0" @default.
- W1539180171 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4211099" @default.
- W1539180171 hasPublicationYear "1974" @default.
- W1539180171 type Work @default.
- W1539180171 sameAs 1539180171 @default.
- W1539180171 citedByCount "54" @default.
- W1539180171 countsByYear W15391801712014 @default.
- W1539180171 crossrefType "journal-article" @default.
- W1539180171 hasAuthorship W1539180171A5010714518 @default.
- W1539180171 hasAuthorship W1539180171A5013759935 @default.
- W1539180171 hasAuthorship W1539180171A5040129865 @default.
- W1539180171 hasBestOaLocation W15391801711 @default.
- W1539180171 hasConcept C108477463 @default.
- W1539180171 hasConcept C181199279 @default.
- W1539180171 hasConcept C185592680 @default.
- W1539180171 hasConcept C2777272437 @default.
- W1539180171 hasConcept C2779856020 @default.
- W1539180171 hasConcept C27911776 @default.
- W1539180171 hasConcept C43617362 @default.
- W1539180171 hasConcept C515207424 @default.
- W1539180171 hasConcept C523546767 @default.
- W1539180171 hasConcept C54355233 @default.
- W1539180171 hasConcept C55493867 @default.
- W1539180171 hasConcept C86803240 @default.
- W1539180171 hasConceptScore W1539180171C108477463 @default.
- W1539180171 hasConceptScore W1539180171C181199279 @default.
- W1539180171 hasConceptScore W1539180171C185592680 @default.
- W1539180171 hasConceptScore W1539180171C2777272437 @default.
- W1539180171 hasConceptScore W1539180171C2779856020 @default.
- W1539180171 hasConceptScore W1539180171C27911776 @default.
- W1539180171 hasConceptScore W1539180171C43617362 @default.
- W1539180171 hasConceptScore W1539180171C515207424 @default.
- W1539180171 hasConceptScore W1539180171C523546767 @default.
- W1539180171 hasConceptScore W1539180171C54355233 @default.
- W1539180171 hasConceptScore W1539180171C55493867 @default.
- W1539180171 hasConceptScore W1539180171C86803240 @default.
- W1539180171 hasLocation W15391801711 @default.
- W1539180171 hasOpenAccess W1539180171 @default.
- W1539180171 hasPrimaryLocation W15391801711 @default.
- W1539180171 hasRelatedWork W131732922 @default.
- W1539180171 hasRelatedWork W1486894952 @default.
- W1539180171 hasRelatedWork W1501839223 @default.
- W1539180171 hasRelatedWork W1530645968 @default.
- W1539180171 hasRelatedWork W1549019539 @default.
- W1539180171 hasRelatedWork W1550256141 @default.
- W1539180171 hasRelatedWork W1550519918 @default.
- W1539180171 hasRelatedWork W1678903690 @default.
- W1539180171 hasRelatedWork W1775749144 @default.
- W1539180171 hasRelatedWork W1912943847 @default.
- W1539180171 hasRelatedWork W1970416250 @default.
- W1539180171 hasRelatedWork W1989423690 @default.
- W1539180171 hasRelatedWork W2004562533 @default.
- W1539180171 hasRelatedWork W2015351127 @default.
- W1539180171 hasRelatedWork W2030302405 @default.
- W1539180171 hasRelatedWork W2032290864 @default.
- W1539180171 hasRelatedWork W2042640333 @default.
- W1539180171 hasRelatedWork W2051832897 @default.
- W1539180171 hasRelatedWork W2092848100 @default.
- W1539180171 hasRelatedWork W3119994227 @default.
- W1539180171 isParatext "false" @default.
- W1539180171 isRetracted "false" @default.
- W1539180171 magId "1539180171" @default.
- W1539180171 workType "article" @default.