Matches in SemOpenAlex for { <https://semopenalex.org/work/W1539292357> ?p ?o ?g. }
- W1539292357 endingPage "24010" @default.
- W1539292357 startingPage "24003" @default.
- W1539292357 abstract "To understand the mechanism of diphtheria toxin membrane translocation, the toxin was entrapped within lipid vesicles, and its low pH-induced translocation across the lipid bilayer was measured. Proteolysis and resistance to guanidinium chloride denaturation were used to demonstrate that the toxin molecules were entrapped. Low pH-induced movement of entrapped toxin to the outer (trans) face of the bilayer was assayed by the binding of external streptavidin to biotin-labeled entrapped toxin. Complete translocation was quantified by the amount of protein released into the external medium. Using whole toxin, it was found that the A fragment was efficiently translocated, but the B fragment was not. This was true both in the low temperature (A domain folded) and high temperature (A domain unfolded) toxin conformations previously identified [Jiang J. X., Abrams, F. S., and London, E. (1991) Biochemistry 30, 3857-3864]. Remarkably, even isolated fragment A appeared to self-translocate under some conditions. Toxin-induced translocation may partly result from formation of a nonspecific toxin-induced pore. This idea is supported by the toxin-induced release of fluorescent dextrans coentrapped within the vesicles. However, low pH-induced exposure of entrapped toxin on the outside of the membrane was conformation dependent. Exposure was greatest for the high temperature conformation. This suggests the existence of a more specific translocation process. The nature and relationship of these processes, and their relative roles in translocation in vivo are discussed." @default.
- W1539292357 created "2016-06-24" @default.
- W1539292357 creator A5031638672 @default.
- W1539292357 creator A5040133867 @default.
- W1539292357 creator A5050766937 @default.
- W1539292357 date "1991-12-01" @default.
- W1539292357 modified "2023-09-26" @default.
- W1539292357 title "Self-translocation of diphtheria toxin across model membranes." @default.
- W1539292357 cites W1486349850 @default.
- W1539292357 cites W1500549143 @default.
- W1539292357 cites W1501842015 @default.
- W1539292357 cites W1506374285 @default.
- W1539292357 cites W1522860483 @default.
- W1539292357 cites W1527208115 @default.
- W1539292357 cites W1539564722 @default.
- W1539292357 cites W1560413344 @default.
- W1539292357 cites W1563060027 @default.
- W1539292357 cites W1563514995 @default.
- W1539292357 cites W1565388437 @default.
- W1539292357 cites W1570872125 @default.
- W1539292357 cites W1572621490 @default.
- W1539292357 cites W157757129 @default.
- W1539292357 cites W1590956479 @default.
- W1539292357 cites W1592027693 @default.
- W1539292357 cites W1592798792 @default.
- W1539292357 cites W1596987991 @default.
- W1539292357 cites W1602413288 @default.
- W1539292357 cites W1604136203 @default.
- W1539292357 cites W1604884879 @default.
- W1539292357 cites W1624904134 @default.
- W1539292357 cites W1896181306 @default.
- W1539292357 cites W1921390505 @default.
- W1539292357 cites W1933755140 @default.
- W1539292357 cites W1966285266 @default.
- W1539292357 cites W1967060446 @default.
- W1539292357 cites W1969920757 @default.
- W1539292357 cites W1974956422 @default.
- W1539292357 cites W1992210902 @default.
- W1539292357 cites W1992950210 @default.
- W1539292357 cites W2003008394 @default.
- W1539292357 cites W2003020350 @default.
- W1539292357 cites W2005950268 @default.
- W1539292357 cites W2005951087 @default.
- W1539292357 cites W2008732666 @default.
- W1539292357 cites W2013185705 @default.
- W1539292357 cites W2016657349 @default.
- W1539292357 cites W2018410651 @default.
- W1539292357 cites W2021078006 @default.
- W1539292357 cites W2027339215 @default.
- W1539292357 cites W2027895029 @default.
- W1539292357 cites W2038119195 @default.
- W1539292357 cites W2065021553 @default.
- W1539292357 cites W2067247972 @default.
- W1539292357 cites W2073308879 @default.
- W1539292357 cites W2080789072 @default.
- W1539292357 cites W2085814385 @default.
- W1539292357 cites W2087733628 @default.
- W1539292357 cites W2091668633 @default.
- W1539292357 cites W2094998867 @default.
- W1539292357 cites W2096490954 @default.
- W1539292357 cites W2137333901 @default.
- W1539292357 cites W21831443 @default.
- W1539292357 cites W2415195591 @default.
- W1539292357 cites W2990934112 @default.
- W1539292357 cites W4247478626 @default.
- W1539292357 cites W4256269761 @default.
- W1539292357 cites W66614937 @default.
- W1539292357 doi "https://doi.org/10.1016/s0021-9258(18)54383-0" @default.
- W1539292357 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1721061" @default.
- W1539292357 hasPublicationYear "1991" @default.
- W1539292357 type Work @default.
- W1539292357 sameAs 1539292357 @default.
- W1539292357 citedByCount "26" @default.
- W1539292357 crossrefType "journal-article" @default.
- W1539292357 hasAuthorship W1539292357A5031638672 @default.
- W1539292357 hasAuthorship W1539292357A5040133867 @default.
- W1539292357 hasAuthorship W1539292357A5050766937 @default.
- W1539292357 hasBestOaLocation W15392923571 @default.
- W1539292357 hasConcept C104317684 @default.
- W1539292357 hasConcept C138626823 @default.
- W1539292357 hasConcept C159047783 @default.
- W1539292357 hasConcept C185592680 @default.
- W1539292357 hasConcept C22070199 @default.
- W1539292357 hasConcept C2777367657 @default.
- W1539292357 hasConcept C2780572238 @default.
- W1539292357 hasConcept C33057221 @default.
- W1539292357 hasConcept C41625074 @default.
- W1539292357 hasConcept C55493867 @default.
- W1539292357 hasConcept C86803240 @default.
- W1539292357 hasConcept C89423630 @default.
- W1539292357 hasConceptScore W1539292357C104317684 @default.
- W1539292357 hasConceptScore W1539292357C138626823 @default.
- W1539292357 hasConceptScore W1539292357C159047783 @default.
- W1539292357 hasConceptScore W1539292357C185592680 @default.
- W1539292357 hasConceptScore W1539292357C22070199 @default.
- W1539292357 hasConceptScore W1539292357C2777367657 @default.
- W1539292357 hasConceptScore W1539292357C2780572238 @default.
- W1539292357 hasConceptScore W1539292357C33057221 @default.