FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1539348717> ?p ?o ?g. }

• W1539348717 endingPage "206" @default.
• W1539348717 startingPage "198" @default.
• W1539348717 abstract "The ciliate Paramecium tetraurelia secretes large amounts of a cysteine protease into the growth medium, presumably for extracellular food digestion. Two endoprotease isozymes (30 and 33 kDa on SDS/PAGE, respectively), both present in cell homogenates and in spent growth medium, were purified to homogeneity. Peptide sequence analysis revealed that these isozymes share identities at the amino acid level but are probably differently processed. Enzymatic characterization of the isolated proteases and sequencing of the cloned cDNA demonstrated that the enzymes belong to the cathepsin-L protease subfamily. Although the identity with mammalian and other protozoan L cathepsins was only around 30%, all important signature sequences for cathepsin L in the preproregion as well as in the catalyst of the enzyme were fully retained. The cDNA of this cysteine protease codes for a preproregion of 108 amino acids. The putative proregion of 86 amino acids which contained the characteristic conserved ERFNIN motif, was fused with a His6 tag, expressed in Escherichia coli, and purified. Both cathepsin L isozymes of Paramecium tetraurelia were inhibited by their cognate propeptide in the nanomolar concentration range. All other cysteine proteases tested (papain and mammalian cathepsin B, G and H) were unaffected by the propeptide up to 10 microM." @default.
• W1539348717 created "2016-06-24" @default.
• W1539348717 creator A5004439886 @default.
• W1539348717 creator A5028133855 @default.
• W1539348717 creator A5037685647 @default.
• W1539348717 creator A5067358405 @default.
• W1539348717 creator A5080130690 @default.
• W1539348717 creator A5081538063 @default.
• W1539348717 creator A5090742074 @default.
• W1539348717 date "1996-05-15" @default.
• W1539348717 modified "2023-09-23" @default.
• W1539348717 title "Cathepsin L is an Intracellular and Extracellular Protease in Paramecium Tetraurelia. Purification, Cloning, Sequencing and Specific Inhibition by its Expressed Propeptide" @default.
• W1539348717 cites W1484675846 @default.
• W1539348717 cites W1535256268 @default.
• W1539348717 cites W1538855357 @default.
• W1539348717 cites W1545290047 @default.
• W1539348717 cites W1581101118 @default.
• W1539348717 cites W1591974475 @default.
• W1539348717 cites W1764993432 @default.
• W1539348717 cites W1932052686 @default.
• W1539348717 cites W1967352806 @default.
• W1539348717 cites W1970640545 @default.
• W1539348717 cites W1976477886 @default.
• W1539348717 cites W1987081227 @default.
• W1539348717 cites W1999808993 @default.
• W1539348717 cites W2000955425 @default.
• W1539348717 cites W2001119936 @default.
• W1539348717 cites W2002444627 @default.
• W1539348717 cites W2004969330 @default.
• W1539348717 cites W2005332386 @default.
• W1539348717 cites W2011899941 @default.
• W1539348717 cites W2017526352 @default.
• W1539348717 cites W2024041934 @default.
• W1539348717 cites W2037041959 @default.
• W1539348717 cites W2040198458 @default.
• W1539348717 cites W2040648848 @default.
• W1539348717 cites W2041923914 @default.
• W1539348717 cites W2042726850 @default.
• W1539348717 cites W2044853699 @default.
• W1539348717 cites W2045373355 @default.
• W1539348717 cites W2050373088 @default.
• W1539348717 cites W2058494069 @default.
• W1539348717 cites W2061163337 @default.
• W1539348717 cites W2075319060 @default.
• W1539348717 cites W2078259381 @default.
• W1539348717 cites W2084815703 @default.
• W1539348717 cites W2086790140 @default.
• W1539348717 cites W2087436968 @default.
• W1539348717 cites W2101025829 @default.
• W1539348717 cites W2163801648 @default.
• W1539348717 cites W2305149595 @default.
• W1539348717 cites W4238170262 @default.
• W1539348717 cites W4321181 @default.
• W1539348717 cites W521619 @default.
• W1539348717 cites W569202338 @default.
• W1539348717 cites W69024186 @default.
• W1539348717 doi "https://doi.org/10.1111/j.1432-1033.1996.0198q.x" @default.
• W1539348717 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8665938" @default.
• W1539348717 hasPublicationYear "1996" @default.
• W1539348717 type Work @default.
• W1539348717 sameAs 1539348717 @default.
• W1539348717 citedByCount "25" @default.
• W1539348717 countsByYear W15393487172015 @default.
• W1539348717 countsByYear W15393487172020 @default.
• W1539348717 crossrefType "journal-article" @default.
• W1539348717 hasAuthorship W1539348717A5004439886 @default.
• W1539348717 hasAuthorship W1539348717A5028133855 @default.
• W1539348717 hasAuthorship W1539348717A5037685647 @default.
• W1539348717 hasAuthorship W1539348717A5067358405 @default.
• W1539348717 hasAuthorship W1539348717A5080130690 @default.
• W1539348717 hasAuthorship W1539348717A5081538063 @default.
• W1539348717 hasAuthorship W1539348717A5090742074 @default.
• W1539348717 hasConcept C104317684 @default.
• W1539348717 hasConcept C119795356 @default.
• W1539348717 hasConcept C145734084 @default.
• W1539348717 hasConcept C153911025 @default.
• W1539348717 hasConcept C159641895 @default.
• W1539348717 hasConcept C167625842 @default.
• W1539348717 hasConcept C167844969 @default.
• W1539348717 hasConcept C174937957 @default.
• W1539348717 hasConcept C181199279 @default.
• W1539348717 hasConcept C182220744 @default.
• W1539348717 hasConcept C187882448 @default.
• W1539348717 hasConcept C2776714187 @default.
• W1539348717 hasConcept C2777283782 @default.
• W1539348717 hasConcept C2779548226 @default.
• W1539348717 hasConcept C2780034444 @default.
• W1539348717 hasConcept C2780412452 @default.
• W1539348717 hasConcept C28021979 @default.
• W1539348717 hasConcept C34695024 @default.
• W1539348717 hasConcept C515207424 @default.
• W1539348717 hasConcept C51618164 @default.
• W1539348717 hasConcept C55493867 @default.
• W1539348717 hasConcept C7860815 @default.
• W1539348717 hasConcept C81216549 @default.
• W1539348717 hasConcept C86803240 @default.
• W1539348717 hasConceptScore W1539348717C104317684 @default.
• W1539348717 hasConceptScore W1539348717C119795356 @default.

• next