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• W1539477643 endingPage "2094" @default.
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• W1539477643 abstract "We have developed and validated a high-performance liquid chromatography method that uses monolithic silica disk-packed spin columns and a monolithic silica column for the simultaneous determination of NG-monomethyl-l-arginine, NG,NG-dimethyl-l-arginine, and NG,NG′-dimethyl-l-arginine in human plasma. For solid-phase extraction, our method employs a centrifugal spin column packed with monolithic silica bonded to propyl benzenesulfonic acid as a cation exchanger. After pretreatment, the methylated arginines are converted to fluorescent derivatives with 4-fluoro-7-nitro-2,1,3-benzoxadiazole, and then the derivatives are separated on a monolithic silica column. l-Arginine concentration was also determined in diluted samples. Standard calibration curves revealed that the assay was linear in the concentration range 0.2–1.0 μM for methylated arginines and 40–200 μM for l-arginine. Linear regression of the calibration curve yielded equations with correlation coefficients of 0.999 (r2). The sensitivity was satisfactory, with a limit of detection ranging from 3.75 to 9.0 fmol for all four compounds. The RSDs were 4.3–4.8% (intraday) and 3.0–6.8% (interday). When this method was applied to samples from six healthy donors, the detected concentrations of NG-monomethyl-l-arginine, NG,NG-dimethyl-l-arginine, NG,NG′-dimethyl-l-arginine and l-arginine were 0.05 ± 0.01, 0.41 ± 0.07, 0.59 ± 0.11, and 83.8 ± 30.43 μM (n = 6), respectively." @default.
• W1539477643 created "2016-06-24" @default.
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• W1539477643 date "2014-06-30" @default.
• W1539477643 modified "2023-10-03" @default.
• W1539477643 title "Simultaneous determination of N G -monomethyl-l -arginine, N G ,N G -dimethyl-l -arginine, N G ,N G′ -dimethyl-l -arginine, and l -arginine using monolithic silica disk-packed spin columns and a monolithic silica column" @default.
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• W1539477643 doi "https://doi.org/10.1002/jssc.201400240" @default.
• W1539477643 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24895248" @default.
• W1539477643 hasPublicationYear "2014" @default.
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