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- W1539608434 abstract "The differentiated human airway epithelium consists of different cell types forming a pseudostratified and polarized epithelium. Basal cells have stem cell properties and can differentiate into columnar, goblet, and ciliated cells. These apically located cells are linked by cell-cell junctions forming the epithelial barrier. It protects the body from inhaled insults, such as cigarette smoke. Defective integrity of the bronchial epithelium potentially contributes to chronic obstructive pulmonary disease (COPD). Further, the airway epithelium in COPD is dramatically altered including basal and goblet cell hyperplasia, as well as squamous cell metaplasia. Here, we analyzed the effect of cigarette smoke extract (CSE) on primary human bronchial epithelial cell (pHBEC) differentiation and barrier formation. pHBEC were differentiated at the air-liquid interface (ALI) into a mucociliary epithelium. Effective differentiation was assessed by immunofluorescence analysis for CC10 (columnar cells), Muc5AC (goblet cells), acetylated tubulin (ciliated cells), and p63 (basal cells). Further, epithelial barrier establishment was confirmed by zonula occludens (ZO)-1 staining as well as transepithelial electrical resistance (TEER) measurements. The exposure to CSE during the differentiation process altered TEER development. Further, CSE delayed tight junction establishment as assessed by ZO-1 staining. Moreover, pHBEC differentiation in the presence of CSE shifted emerging cell population numbers including delayed appearance of ciliated cells. Our data suggest that chronic exposure to CSE during pHBEC differentiation at the ALI provides a suitable tool to study airway epithelial changes occurring in COPD." @default.
- W1539608434 created "2016-06-24" @default.
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- W1539608434 date "2014-09-01" @default.
- W1539608434 modified "2023-09-26" @default.
- W1539608434 title "Cigarette smoke affects differentiation and function of primary human bronchial epithelial cells" @default.
- W1539608434 hasPublicationYear "2014" @default.
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