Matches in SemOpenAlex for { <https://semopenalex.org/work/W1539688758> ?p ?o ?g. }
- W1539688758 endingPage "6452" @default.
- W1539688758 startingPage "6445" @default.
- W1539688758 abstract "Glucosidase I initiates the processing of asparagine-linked glycoproteins by excising the distal alpha 1,2-linked glucosyl residue from the Glc3Man9GlcNAc2 oligosaccharide, soon after its en bloc transfer from the lipid-linked donor to the nascent polypeptide. 1-Deoxynojirimycin, an analog of D-glucose, is a potent competitive inhibitor of the enzyme. Sulfhydryl-seeking reagents also strongly inhibit the enzyme, implying the involvement of an -SH group in its activity. To test this hypothesis, glucosidase I was purified from the rat mammary gland and its active site was loaded with 1-deoxynojirimycin, to protect such a group(s), while -SH groups on the remaining surface of the enzyme were blocked with N-ethylmaleimide or para-chloromercuriphenylsulfonic acid. Deoxynojirimycin was removed by dialysis to expose the active site -SH group(s). This group(s) was then tagged with 3-(N-maleimidopropionyl)biocytin (MPB) and detected with 125I-streptavidin on Western blots. A series of experiments is presented to show that indeed a critical -SH group(s) is located within the catalytic site of the enzyme. Additionally, the enzyme also possesses one or more sulfhydryls and disulfide bonds in its primary structure. The experimental approach outlined here should apply to identify reactive sulfhydryl groups in other catalytically active proteins." @default.
- W1539688758 created "2016-06-24" @default.
- W1539688758 creator A5028933167 @default.
- W1539688758 creator A5069076350 @default.
- W1539688758 creator A5089366564 @default.
- W1539688758 date "1993-03-01" @default.
- W1539688758 modified "2023-10-16" @default.
- W1539688758 title "Role of sulfhydryl groups in the function of glucosidase I from mammary gland." @default.
- W1539688758 cites W116794318 @default.
- W1539688758 cites W128307323 @default.
- W1539688758 cites W1485013234 @default.
- W1539688758 cites W1487347701 @default.
- W1539688758 cites W1489462168 @default.
- W1539688758 cites W1490548775 @default.
- W1539688758 cites W1500163587 @default.
- W1539688758 cites W1502371571 @default.
- W1539688758 cites W1503953699 @default.
- W1539688758 cites W1508695353 @default.
- W1539688758 cites W1512614115 @default.
- W1539688758 cites W1513057329 @default.
- W1539688758 cites W1520564498 @default.
- W1539688758 cites W1522309084 @default.
- W1539688758 cites W1523026250 @default.
- W1539688758 cites W1524106947 @default.
- W1539688758 cites W1525457441 @default.
- W1539688758 cites W1525924495 @default.
- W1539688758 cites W1527433663 @default.
- W1539688758 cites W1539740676 @default.
- W1539688758 cites W1541926167 @default.
- W1539688758 cites W1542297760 @default.
- W1539688758 cites W1543064002 @default.
- W1539688758 cites W1543885109 @default.
- W1539688758 cites W1546588955 @default.
- W1539688758 cites W1553410372 @default.
- W1539688758 cites W1559526379 @default.
- W1539688758 cites W1561708917 @default.
- W1539688758 cites W1565917945 @default.
- W1539688758 cites W1568607294 @default.
- W1539688758 cites W1574752691 @default.
- W1539688758 cites W1575935170 @default.
- W1539688758 cites W1580158514 @default.
- W1539688758 cites W1584526531 @default.
- W1539688758 cites W1586039957 @default.
- W1539688758 cites W1588995938 @default.
- W1539688758 cites W1592482289 @default.
- W1539688758 cites W1593647457 @default.
- W1539688758 cites W1599589896 @default.
- W1539688758 cites W1606121585 @default.
- W1539688758 cites W1614268180 @default.
- W1539688758 cites W1642778195 @default.
- W1539688758 cites W1643469489 @default.
- W1539688758 cites W1647338923 @default.
- W1539688758 cites W1659796181 @default.
- W1539688758 cites W1861997264 @default.
- W1539688758 cites W1946018878 @default.
- W1539688758 cites W1975816899 @default.
- W1539688758 cites W1982826235 @default.
- W1539688758 cites W1987216283 @default.
- W1539688758 cites W1990212916 @default.
- W1539688758 cites W2003645640 @default.
- W1539688758 cites W2006548681 @default.
- W1539688758 cites W2006613155 @default.
- W1539688758 cites W2014798355 @default.
- W1539688758 cites W2031313147 @default.
- W1539688758 cites W2038570018 @default.
- W1539688758 cites W2059373310 @default.
- W1539688758 cites W2064379748 @default.
- W1539688758 cites W2067110717 @default.
- W1539688758 cites W2068161396 @default.
- W1539688758 cites W2077044755 @default.
- W1539688758 cites W2080793734 @default.
- W1539688758 cites W2087392513 @default.
- W1539688758 cites W2088599228 @default.
- W1539688758 cites W2100837269 @default.
- W1539688758 cites W2119560646 @default.
- W1539688758 cites W2152708819 @default.
- W1539688758 cites W2165036715 @default.
- W1539688758 cites W2335825284 @default.
- W1539688758 cites W4206701382 @default.
- W1539688758 cites W4236230521 @default.
- W1539688758 doi "https://doi.org/10.1016/s0021-9258(18)53272-5" @default.
- W1539688758 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8454617" @default.
- W1539688758 hasPublicationYear "1993" @default.
- W1539688758 type Work @default.
- W1539688758 sameAs 1539688758 @default.
- W1539688758 citedByCount "11" @default.
- W1539688758 countsByYear W15396887582014 @default.
- W1539688758 countsByYear W15396887582015 @default.
- W1539688758 crossrefType "journal-article" @default.
- W1539688758 hasAuthorship W1539688758A5028933167 @default.
- W1539688758 hasAuthorship W1539688758A5069076350 @default.
- W1539688758 hasAuthorship W1539688758A5089366564 @default.
- W1539688758 hasBestOaLocation W15396887581 @default.
- W1539688758 hasConcept C121608353 @default.
- W1539688758 hasConcept C126322002 @default.
- W1539688758 hasConcept C134018914 @default.
- W1539688758 hasConcept C14036430 @default.
- W1539688758 hasConcept C185592680 @default.