FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1539795210> ?p ?o ?g. }

• W1539795210 endingPage "5809" @default.
• W1539795210 startingPage "5800" @default.
• W1539795210 abstract "High-mobility group box 1 protein (HMGB1) has been studied as a key mediator of inflammatory diseases, including sepsis. Regulating secretion is important in the control of HMGB1-mediated inflammation. Previously, it was shown that HMGB1 needs to be phosphorylated for secretion. In this study, we show that HMGB1 is phosphorylated by the classical protein kinase C (cPKC) and is secreted by a calcium-dependent mechanism. For this study, RAW264.7 cells and human peripheral blood monocytes were treated with PI3K inhibitors wortmannin, LY294002, and ZSTK474, resulting in inhibition of LPS-stimulated HMGB1 secretion, whereas inhibitors of NF-kappaB and MAPKs p38 and ERK showed no inhibition. Akt inhibitor IV and mammalian target of rapamycin inhibitor rapamycin did not inhibit HMGB1 secretion. However, the PKC inhibitors Gö6983 (broad-spectrum PKC), Gö6976 (cPKC), and Ro-31-7549 (cPKC) and phosphoinositide-dependent kinase 1 inhibitor, which results in protein kinase C (PKC) inhibition, inhibited LPS-stimulated HMGB1 secretion. PKC activators, PMA and bryostatin-1, enhanced HMGB1 secretion. In an in vitro kinase assay, HMGB1 was phosphorylated by recombinant cPKC and by purified nuclear cPKC from LPS-stimulated RAW264.7 cells, but not by casein kinase II or cdc2. HMGB1 secretion was also induced by the calcium ionophore A23187 and inhibited by the Ca(2+) chelators BAPTA-AM and EGTA. These findings support a role for Ca(2+)-dependent PKC in HMGB1 secretion. Thus, we propose that cPKC is an effector kinase of HMGB1 phosphorylation in LPS-stimulated monocytes and PI3K-phosphoinositide-dependent kinase 1 may act in concert to control HMGB1 secretion independent of the NF-kappaB, p38, and ERK pathways." @default.
• W1539795210 created "2016-06-24" @default.
• W1539795210 creator A5000708221 @default.
• W1539795210 creator A5002353799 @default.
• W1539795210 creator A5012713592 @default.
• W1539795210 creator A5026330462 @default.
• W1539795210 creator A5035133412 @default.
• W1539795210 creator A5050662142 @default.
• W1539795210 creator A5063274572 @default.
• W1539795210 date "2009-05-01" @default.
• W1539795210 modified "2023-09-30" @default.
• W1539795210 title "HMGB1 Is Phosphorylated by Classical Protein Kinase C and Is Secreted by a Calcium-Dependent Mechanism" @default.
• W1539795210 cites W1490007860 @default.
• W1539795210 cites W1542062417 @default.
• W1539795210 cites W1550722396 @default.
• W1539795210 cites W1568761178 @default.
• W1539795210 cites W1580291739 @default.
• W1539795210 cites W1580679843 @default.
• W1539795210 cites W1590680361 @default.
• W1539795210 cites W1605863240 @default.
• W1539795210 cites W1606913060 @default.
• W1539795210 cites W1627944730 @default.
• W1539795210 cites W1631475273 @default.
• W1539795210 cites W1641731911 @default.
• W1539795210 cites W1822066871 @default.
• W1539795210 cites W1909011356 @default.
• W1539795210 cites W1934726430 @default.
• W1539795210 cites W1963590275 @default.
• W1539795210 cites W1967459506 @default.
• W1539795210 cites W1973844063 @default.
• W1539795210 cites W1980814667 @default.
• W1539795210 cites W1998762942 @default.
• W1539795210 cites W2003542500 @default.
• W1539795210 cites W2004266769 @default.
• W1539795210 cites W2010817360 @default.
• W1539795210 cites W2023427346 @default.
• W1539795210 cites W2027731275 @default.
• W1539795210 cites W2029068688 @default.
• W1539795210 cites W2041797033 @default.
• W1539795210 cites W2041926656 @default.
• W1539795210 cites W2042821764 @default.
• W1539795210 cites W2046525768 @default.
• W1539795210 cites W2059038780 @default.
• W1539795210 cites W2074294483 @default.
• W1539795210 cites W2081449594 @default.
• W1539795210 cites W2081598827 @default.
• W1539795210 cites W2085495891 @default.
• W1539795210 cites W2090407808 @default.
• W1539795210 cites W2091213087 @default.
• W1539795210 cites W2096931514 @default.
• W1539795210 cites W2100576517 @default.
• W1539795210 cites W2104150310 @default.
• W1539795210 cites W2106039427 @default.
• W1539795210 cites W2109519875 @default.
• W1539795210 cites W2120138780 @default.
• W1539795210 cites W2131392583 @default.
• W1539795210 cites W2143250379 @default.
• W1539795210 cites W2143945587 @default.
• W1539795210 cites W2146157811 @default.
• W1539795210 cites W2146989813 @default.
• W1539795210 cites W2155813411 @default.
• W1539795210 cites W2161798777 @default.
• W1539795210 cites W2164171309 @default.
• W1539795210 cites W2164714222 @default.
• W1539795210 cites W2171028311 @default.
• W1539795210 cites W2317425438 @default.
• W1539795210 cites W4233770333 @default.
• W1539795210 cites W4313344700 @default.
• W1539795210 doi "https://doi.org/10.4049/jimmunol.0801873" @default.
• W1539795210 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19380828" @default.
• W1539795210 hasPublicationYear "2009" @default.
• W1539795210 type Work @default.
• W1539795210 sameAs 1539795210 @default.
• W1539795210 citedByCount "141" @default.
• W1539795210 countsByYear W15397952102012 @default.
• W1539795210 countsByYear W15397952102013 @default.
• W1539795210 countsByYear W15397952102014 @default.
• W1539795210 countsByYear W15397952102015 @default.
• W1539795210 countsByYear W15397952102016 @default.
• W1539795210 countsByYear W15397952102017 @default.
• W1539795210 countsByYear W15397952102018 @default.
• W1539795210 countsByYear W15397952102019 @default.
• W1539795210 countsByYear W15397952102020 @default.
• W1539795210 countsByYear W15397952102021 @default.
• W1539795210 countsByYear W15397952102022 @default.
• W1539795210 countsByYear W15397952102023 @default.
• W1539795210 crossrefType "journal-article" @default.
• W1539795210 hasAuthorship W1539795210A5000708221 @default.
• W1539795210 hasAuthorship W1539795210A5002353799 @default.
• W1539795210 hasAuthorship W1539795210A5012713592 @default.
• W1539795210 hasAuthorship W1539795210A5026330462 @default.
• W1539795210 hasAuthorship W1539795210A5035133412 @default.
• W1539795210 hasAuthorship W1539795210A5050662142 @default.
• W1539795210 hasAuthorship W1539795210A5063274572 @default.
• W1539795210 hasBestOaLocation W15397952101 @default.
• W1539795210 hasConcept C11960822 @default.
• W1539795210 hasConcept C153911025 @default.
• W1539795210 hasConcept C170493617 @default.

• next