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- W1539885989 abstract "Objective To develop a rapid and simple technology for the detection of Peste des petits ruminants virus (PPRV). Methods Two pairs of primers and a set of loop primer targeting the N gene of PPRV were designed. The concentration of Mg2+, Betaine, Bst DNA Polymerase, dNTP, loop primer, temperature and other conditions were optimized. Results The amplification was carried out in a single tube at 62 ℃, and the amplification results were visualized within 30 min. This test has a high specificity for PPRV detection, and the minimum limit detected was 1.6 TCID50 virus. Conclusion The sensitive and simple LAMP assay is a potential and useful technique for PPRV detection." @default.
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- W1539885989 date "2009-01-01" @default.
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- W1539885989 title "Development of loop-mediated isothermal amplification (LAMP) method for the detection of peste des petits ruminants virus." @default.
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